ISOLATION AND CHARACTERIZATION OF A HIGH-MOLECULAR-WEIGHT TYPE-IV COLLAGENASE ISOLATED FROM HUMAN CARCINOMA TISSUE

被引：8

作者：

TSUDA, TT ^{[1
]}

KODAMA, A ^{[1
]}

YAMAMURA, M ^{[1
]}

MATSUZAKI, S ^{[1
]}

TSUDA, M ^{[1
]}

机构：

[1] BML INC,CTR R&D,SAITAMA 350,JAPAN

来源：

FEBS LETTERS | 1993年 / 319卷 / 1-2期

关键词：

HIGH MOLECULAR WEIGHT TYPE-IV COLLAGENASE; TYPE-IV COLLAGENASE; METASTATIC CARCINOMA TISSUE;

D O I：

10.1016/0014-5793(93)80032-P

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A proform of high molecular weight type IV collagenase was isolated and purified 1230-fold from human metastatic carcinoma tissue. Like matrix metalloproteinases (MMPs), the enzyme was activated by trypsin and degraded type IV collagen and gelatin at a neutral pH, the activity was inhibited by EDTA and o-phenanthroline. However, the molecular weight was much higher than MMPs which degraded type IV collagen, gelatinase A (MMP-2; 72 kDa gelatinase/type IV collagenase) (EC 3.4.24.24), gelatinase B (MMP-9; 92 kDa gelatinase/type IV collagenase) (EC 3.4.24.35), stromelysin-1 (MMP-3; 57 kDa) (EC 3.4.24.17) and stromelysin-2 (MMP-10; 57 kDa) (EC 3.4.24.22). The other significant difference from MMPs was that the enzyme was not activated by 4-aminophenylmercuric acetate nor inhibited by TIMP. Taking together these results, this high molecular weight type IV collagenase might be a newly found enzyme different from MMPs or might have the same configuration as MMPs already reported.

引用

页码：35 / 39

页数：5

共 28 条

[1] RAS ONCOGENE MEDIATED INDUCTION OF A 92KDA METALLOPROTEINASE - STRONG CORRELATION WITH THE MALIGNANT PHENOTYPE [J].