PROBING DNA TRIPLE HELIX STRUCTURE BY CHEMICAL LIGATION

被引：7

作者：

DOLINNAYA, NG

PYATRAUSKENE, OV

SHABAROVA, ZA

机构：

[1] AN BELOZERSKII MOLEC BIOL & BIOORGAN CHEM LAB,MOSCOW,USSR

[2] MV LOMONOSOV STATE UNIV,DEPT CHEM,MOSCOW 119899,USSR

来源：

FEBS LETTERS | 1991年 / 284卷 / 02期

关键词：

DNA TRIPLEX; CHEMICAL LIGATION OF NUCLEIC ACIDS;

D O I：

10.1016/0014-5793(91)80692-V

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A series of oligomeric double and triple helical DNAs with irregular sequences of homopurine and homopyrimidine strands were prepared. DNA triplexes were identified by CD spectroscopy and thermal denaturation profiles (biphasic helix-coil transition). Condensation of oligonucleotides on single and double-stranded DNA templates was performed using water-soluble carbodiimide, phosphodiester and pyrophosphate internucleotide bonds being newly formed. Such chemical ligation proved to be a sensitive monitor of changes in the sugar-phosphate backbone resulting from conversion of double to triple helix and of third-strand binding.

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页码：232 / 234

页数：3

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