THE TGN38 GLYCOPROTEIN CONTAINS 2 NONOVERLAPPING SIGNALS THAT MEDIATE LOCALIZATION TO THE TRANS-GOLGI NETWORK

被引：132

作者：

PONNAMBALAM, S

RABOUILLE, C

LUZIO, JP

NILSSON, T

WARREN, G

机构：

[1] IMPERIAL CANC RES FUND,CELL BIOL LAB,LINCOLNS INNS FIELDS,LONDON WC2A 3PX,ENGLAND

[2] UNIV CAMBRIDGE,ADDENBROOKES HOSP,DEPT CLIN BIOCHEM,CAMBRIDGE CB2 2QR,ENGLAND

来源：

JOURNAL OF CELL BIOLOGY | 1994年 / 125卷 / 02期

关键词：

D O I：

10.1083/jcb.125.2.253

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The membrane-spanning and cytoplasmic domains of CD4 and CD8 were replaced by those of TGN38. After transient expression in HeLa cells, the location of the hybrid proteins was determined using immunofluorescence and quantitative immuno-electron microscopy, FACS analysis and metabolic labeling. The membrane-spanning domain was found to contain a signal that localized hybrid proteins to the TGN. This was in addition to the signal previously identified in the cytoplasmic domain (Bos, K., C. Wraight, and K. Stanley. 1993. EMBO (Eur. Mol. Biol. Organ) J. 12:2219-2228. Humphrey, J. S., P. J. Peters, L. C. Yuan, and J. S. Bonifacino. 1993. J. Cell Biol. 120:1123-1135. Wong, S. H., and W. Hong. 1993. J. Biol. Chem. 268:22853-22862). The different properties of these two signals suggest that each operates by a different mechanism.

引用

页码：253 / 268

页数：16

共 71 条

[1] GOLGI RETENTION OF A TRANS-GOLGI MEMBRANE-PROTEIN, GALACTOSYL-TRANSFERASE, REQUIRES CYSTEINE AND HISTIDINE-RESIDUES WITHIN THE MEMBRANE-ANCHORING DOMAIN [J].