ANTICANCER ACTIVITY OF FREE GAMMA-LINOLENIC ACID ON AH-109A RAT HEPATOMA-CELLS AND THE EFFECT OF SERUM-ALBUMIN ON ANTICANCER ACTIVITY OF GAMMA-LINOLENIC ACID INVITRO

被引：19

作者：

HAYASHI, Y ^{[1
]}

FUKUSHIMA, S ^{[1
]}

HIRATA, T ^{[1
]}

KISHIMOTO, S ^{[1
]}

KATSUKI, T ^{[1
]}

NAKANO, M ^{[1
]}

机构：

[1] KUMAMOTO UNIV,SCH MED,DEPT MICROBIOL,KUMAMOTO 860,JAPAN

来源：

JOURNAL OF PHARMACOBIO-DYNAMICS | 1990年 / 13卷 / 11期

关键词：

ALPHA-LINOLENIC ACID; CYTOTOXICITY; HEPATOMA CELL; ALBUMIN; LIPID PEROXIDATION; INVITRO STUDY;

D O I：

10.1248/bpb1978.13.705

中图分类号：

R9 [药学];

学科分类号：

1007 [药学];

摘要：

The cytotoxicity of gamma-linolenic acid (C18:3n-6) against rat hepatoma AH-109A cells and the effect of bovine serum albumin (BSA) on its toxicity were examined in culture. The proliferation of AH-109A cells, evaluated by the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay, was significantly suppressed by gamma-linolenic acid above 5-mu-g/ml concentration in a serum-free culture medium. However, its toxicity was reduced by supplement of BSA. Similar observation of reduced toxicity by BSA was shown by the method of trypan blue dye exclusion and a colony formation assay. The cytotoxicity of gamma-linolenic acid was correlated closely with the concentration of unbound (free) gamma-linolenic acid. Production of thiobarbituric acid reactive material, one of the indicators of lipid peroxidation, was stimulated by gamma-linolenic acid and inhibited by BSA. These results suggested that the presence of albumin suppressed the cytotoxicity of the free fatty acid.

引用

页码：705 / 711

页数：7

共 28 条

[1]

BADWEY JA, 1984, J BIOL CHEM, V259, P7870

[2]

BEGIN ME, 1986, ANTICANCER RES, V6, P291

[3]

BEGIN ME, 1986, JNCI-J NATL CANCER I, V77, P1053

[4]

POLY-UNSATURATED FATTY ACID-INDUCED CYTO-TOXICITY AGAINST TUMOR-CELLS AND ITS RELATIONSHIP TO LIPID-PEROXIDATION [J].

BEGIN, ME ;

ELLS, G ;

HORROBIN, DF .

JOURNAL OF THE NATIONAL CANCER INSTITUTE, 1988, 80 (03) :188-194

[5]

SELECTIVE KILLING OF HUMAN CANCER-CELLS BY POLY-UNSATURATED FATTY-ACIDS [J].

BEGIN, ME ;

DAS, UN ;

ELLS, G ;

HORROBIN, DF .

PROSTAGLANDINS LEUKOTRIENES AND MEDICINE, 1985, 19 (02) :177-186

[6]

BOOYENS J, 1984, S AFR MED J, V65, P240

[7]

DIETARY POLYUNSATURATED FAT IN RELATION TO MAMMARY CARCINOGENESIS IN RATS [J].

BRADEN, LM ;

CARROLL, KK .

LIPIDS, 1986, 21 (04) :285-288

[8]

CANNIZZO F, 1989, CANCER RES, V49, P4289

[9]

SUPPRESSION OF GROWTH IN A LEUKEMIC T-CELL LINE BY N-3 AND N-6 POLY-UNSATURATED FATTY-ACIDS [J].

CHOW, SC ;

SISFONTES, L ;

BJORKHEM, I ;

JONDAL, M .

LIPIDS, 1989, 24 (08) :700-704

[10]

DOI O, 1978, BIOCHIM BIOPHYS ACTA, V509, P239, DOI 10.1016/0005-2736(78)90044-5

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