THE MODELING AND ANALYSIS OF THE ELUTION STAGE OF BIOSPECIFIC ADSORPTION IN FIXED-BEDS

A mathematical model that describes the dynamic behavior of the elution stage of biospecific adsorption (affinity chromatography) in a fixed bed is developed and solved. Both non-selective and selective elution methods are considered. The results show that the duration of the elution stage for a given bed length decreases as the value of the Porath parameter for elution increases. The concentrating effect of the elution stage on the adsorbate of interest increases as the bed length and the value of the Porath parameter for elution increase. The effect of direction of flow during elution is studied and the advantages with a reversed flow are described.