A GAS-CHROMATOGRAPHIC PROCEDURE FOR SEPARATION AND QUANTITATION OF THE ENANTIOMERS OF THE ANTIDEPRESSANT TRANYLCYPROMINE

被引：7

作者：

ASPESLET, LJ

BAKER, GB

COUTTS, RT

MOUSSEAU, DD

机构：

[1] UNIV ALBERTA,DEPT PSYCHIAT,NEUROCHEM RES UNIT,EDMONTON T6G 2B7,ALBERTA,CANADA

[2] UNIV ALBERTA,FAC PHARM & PHARMACEUT SCI,EDMONTON T6G 2B7,ALBERTA,CANADA

来源：

BIOCHEMICAL PHARMACOLOGY | 1992年 / 44卷 / 09期

关键词：

D O I：

10.1016/0006-2952(92)90088-Z

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

A novel assay procedure has been developed that allows for the separation and quantification of the enantiomers of the monoamine oxidase inhibitor tranylcypromine (TCP) in brain and liver of rats. The analytical method involves extraction of the drug from rat tissue with an organic solvent. TCP is then derivatized with S-(-)-N-(trifluoroacetyl)-prolyl chloride to allow gas chromatographic analysis of the resulting diastereoisomers. Conditions for analysis by a gas chromatograph equipped with a nitrogen-phosphorus detector and a capillary column are described. The method has been applied to the separation and quantification of the enantiomers of TCP in samples of brain and liver of rats that had been injected with this drug alone and after pretreatment with iprindole, a drug known to block aromatic ring hydroxylation.

引用

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页码：1894 / 1897

页数：4

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