DEVELOPMENTAL DIFFERENCES IN RENAL SULFATE REABSORPTION - TRANSPORT KINETICS IN BRUSH-BORDER MEMBRANE-VESICLES

被引：9

作者：

PENA, DR

NEIBERGER, RE

机构：

[1] Department of Pediatrics, JHMHC, University of Florida College of Medicine, Gainesville, 32610-0296, FL

来源：

PEDIATRIC NEPHROLOGY | 1992年 / 6卷 / 06期

关键词：

RENAL DEVELOPMENT; RENAL SULFATE TRANSPORT; BRUSH BORDER MEMBRANE VESICLES; TRANSPORT; RENAL PHYSIOLOGY;

D O I：

10.1007/BF00866495

中图分类号：

R72 [儿科学];

学科分类号：

100202 ;

摘要：

Renal tubular reabsorption of inorganic sulfate is greater in younger than older guinea pigs. To determine the mechanism of this difference, we studied the transport of inorganic sulfate in renal brush border membrane vesicles (BBMV) obtained from young (<25 days) and adult guinea pigs (>60 days). BBMV were obtained by mechanical and osmotic disruption of dissected renal cortices followed by magnesium precipitation and differential centrifugation. After the membranes were incubated for 10 s in solutions containing inorganic sulfate at several concentrations (0.1-10 mM) and trace amounts of sulfate-35, intravesicular uptake was measured. Based on sulfur-35 uptake, reabsorption transport kinetics (V(max) and K(m)) were estimated. BBMV obtained from young guinea pigs demonstrated higher sodium-sulfate cotransport, V(max) (51.79+/-4.34 pmol/mg protein per s) than those obtained from adult animals (V(max) = 34.28+/-9.17 pmol/mg per s), P <0.05. V(max) values are represented as means plus or minus standard deviation. No differences in K(m) were observed. Our results indicate that age-related differences in renal inorganic sulfate reabsorption are due to a higher V(max) for sodium-sulfate cotransport in the younger animals, suggesting a higher density of sodium-sulfate cotransporters or an increased cotransport turnover rate in this age group.

引用

页码：532 / 535

页数：4

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