SUCROSE SYNTHASE GENES IN BARLEY - CDNA CLONING OF THE SS2 TYPE AND TISSUE-SPECIFIC EXPRESSION OF SS1 AND SS2

被引：24

作者：

DEILARDUYA, OM ^{[1
]}

VICENTECARBAJOSA, J ^{[1
]}

DELAHOZ, PS ^{[1
]}

CARBONERO, P ^{[1
]}

机构：

[1] UPM,ETS INGN AGRON,CATEDRA BIOQUIM & BIOL MOLEC,CIUDAD UNIV,E-28040 MADRID,SPAIN

来源：

FEBS LETTERS | 1993年 / 320卷 / 02期

关键词：

BARLEY; CDNA CLONING; GENE EXPRESSION; NORTHERN BLOT; SPECIFIC PROBE; SUCROSE SYNTHASE GENE;

D O I：

10.1016/0014-5793(93)80087-B

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cDNA of 2,708 bp encoding type 2 sucrose synthase (Ss2) from barley has been sequenced. Similarity of this cDNA with respect to that of type 1 (Ss1) is high in the coding region (75% identical positions), but low (41% identical residues) in the 3' non-coding region. Type-specific non cross-hybridizing probes for Northern blot analysis have been obtained from the 3' ends. The Ss1 type is highly expressed in developing endosperm and in roots and, at lower levels, in coleoptiles and aleurone. The Ss2 mRNA is abundant in endosperm, low in aleurone, and undetected in coleoptiles and roots.

引用

页码：177 / 181

页数：5

共 15 条

[1]

ARAI M, 1992, PLANT CELL PHYSIOL, V33, P503

[2]

BURR B, 1981, GENETICS, V98, P143

[3]

CARDINI C. E., 1955, JOUR BIOL CHEM, V214, P149

[4] SUCROSE SYNTHASE OF ARABIDOPSIS - GENOMIC CLONING AND SEQUENCE CHARACTERIZATION [J].

CHOPRA, S ;

DELFAVERO, J ;

DOLFERUS, R ;

JACOBS, M .

PLANT MOLECULAR BIOLOGY, 1992, 18 (01) :131-134

[5] HOMOLOGOUS SUCROSE SYNTHASE GENES IN BARLEY (HORDEUM-VULGARE) ARE LOCATED IN CHROMOSOMES 7H (SYN-1) AND 2H - EVIDENCE FOR A GENE TRANSLOCATION [J].

DELAHOZ, PS ;

VICENTECARBAJOSA, J ;

MENA, M ;

CARBONERO, P .

FEBS LETTERS, 1992, 310 (01) :46-50

[6] A COMPARISON OF 2 SUCROSE SYNTHETASE ISOZYMES FROM NORMAL AND SHRUNKEN-1 MAIZE [J].

ECHT, CS ;

CHOUREY, PS .

PLANT PHYSIOLOGY, 1985, 79 (02) :530-536

[7] GENOMIC CLONES OF A WILD-TYPE ALLELE AND A TRANSPOSABLE ELEMENT-INDUCED MUTANT ALLELE OF THE SUCROSE SYNTHASE GENE OF ZEA-MAYS-L [J].

GEISER, M ;

WECK, E ;

DORING, HP ;

WERR, W ;

COURAGETEBBE, U ;

TILLMANN, E ;

STARLINGER, P .

EMBO JOURNAL, 1982, 1 (11) :1455-1460

[8] ALPHA-AMYLASE GENES OF WHEAT ARE 2 MULTIGENE FAMILIES WHICH ARE DIFFERENTIALLY EXPRESSED [J].

LAZARUS, CM ;

BAULCOMBE, DC ;

MARTIENSSEN, RA .

PLANT MOLECULAR BIOLOGY, 1985, 5 (01) :13-24

[9] LINKED SUCROSE SYNTHASE GENES IN GROUP-7 CHROMOSOMES IN HEXAPLOID WHEAT (TRITICUM-AESTIVUM L) [J].

MARANA, C ;

GARCIAOLMEDO, F ;

CARBONERO, P .

GENE, 1988, 63 (02) :253-260

[10] DIFFERENTIAL EXPRESSION OF 2 TYPES OF SUCROSE SYNTHASE-ENCODING GENES IN WHEAT IN RESPONSE TO ANAEROBIOSIS, COLD SHOCK AND LIGHT [J].

MARANA, C ;

GARCIAOLMEDO, F ;

CARBONERO, P .

GENE, 1990, 88 (02) :167-172

← 1 2 →