REFINED SOLUTION STRUCTURE OF A DNA HETERODUPLEX CONTAINING AN ALDEHYDIC ABASIC SITE

被引：127

作者：

GOLJER, I ^{[1
]}

KUMAR, S ^{[1
]}

BOLTON, PH ^{[1
]}

机构：

[1] WESLEYAN UNIV,DEPT CHEM,MIDDLETOWN,CT 06459

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 1995年 / 270卷 / 39期

关键词：

D O I：

10.1074/jbc.270.39.22980

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The solution structure of the DNA duplex d(C(1)G(2)C(3)G(4) A(5)D(6)A(7)C(8)G(9)C(10)C(11))-d(G(22)C(21)G(20)C(19)T(18)A(17)T(16)G(15)C(14)G(13)G(12)), with D indicating a deoxyribose aldehyde abasic site and numbering from 5' to 3', has been determined by the combined use of NMR and restrained molecular dynamics. The P-31 and P-31-H-1 correlation data indicate that the backbones of these duplex DNAs are regular. One- and two-dimensional H-1 NMR data indicate that the duplexes are right-handed and B-form. Conformational changes due to the presence of the abasic site extends to the base pairs adjacent to the lesion site with the local conformation of the DNA being dependent on whether the abasic site is in the alpha or beta configuration. When the sugar of the abasic site is in the beta configuration the deoxyribose is within the helix, whereas when the sugar is in the cu configuration the deoxyribose is out of the helix. The base of residue A(17) in the position opposite the abasic site is predominantly stacked in the helix in both cases. A water molecule can apparently form a hydrogen bond bridge between the beta abasic site and A(17).

引用

页码：22980 / 22987

页数：8

共 53 条

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