RESTRICTION SITE POLYMORPHISM OF THE GENES ENCODING THE MAJOR 25 KDA AND 36 KDA OUTER-MEMBRANE PROTEINS OF BRUCELLA

被引:133
作者
CLOECKAERT, A
VERGER, JM
GRAYON, M
GREPINET, O
机构
[1] Lab Pathologie Infectieuse Immunolog, Inst National Recherche Agronomique
来源
MICROBIOLOGY-UK | 1995年 / 141卷
关键词
BRUCELLA; OUTER-MEMBRANE PROTEINS; POLYMORPHISM;
D O I
10.1099/13500872-141-9-2111
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Seventy-seven Brucella reference and field strains from different geographic origins and hosts representing the six recognized species and their different biovars were analysed for diversity of their genes encoding the major 25 and 36 kDa outer-membrane proteins (OMPs) by PCR-RFLP. The 25 kDa OMP is encoded by a single gene (omp25) whereas two closely related genes (omp2a and omp2b) encode and potentially express the 36 kDa OMP. Analysis of PCR products of the omp25 gene digested with nine restriction enzymes revealed two species-specific markers, i.e. the absence of the EcoRV site in all Brucella melitensis strains and an similar to 50 bp deletion at the 3' terminal end of the gene in all Brucella ovis strains. Analysis of PCR products of the omp2a and omp2b genes digested with 13 restriction enzymes indicated a greater diversity than the omp25 gene among the six Brucella species and within the Brucella abortus, Brucella suis, B. melitensis and B. ovis species. Greater polymorphism was also detected for the omp2b than for the omp2a gene, especially in B. ovis which seemed to carry two similar (but not identical) copies of omp2a instead of one copy each of omp2a and omp2b for the other Brucella species as was previously suggested by Ficht et al. (1990; Mol Microbiol 4, 1135-1142). Results of PCR-RFLP indicated that distinction can be made between Brucella species and some of their biovars, except between B. canis and B. suis by. 3 and 4, on the basis of the size and diversity of their major OMP genes, and that it could be of importance for diagnostic, epidemiological and evolutionary study purposes.
引用
收藏
页码:2111 / 2121
页数:11
相关论文
共 26 条
[1]   DNA POLYMORPHISM IN STRAINS OF THE GENUS BRUCELLA [J].
ALLARDETSERVENT, A ;
BOURG, G ;
RAMUZ, M ;
PAGES, M ;
BELLIS, M ;
ROIZES, G .
JOURNAL OF BACTERIOLOGY, 1988, 170 (10) :4603-4607
[2]  
Alton G.G., 1988, TECHNIQUES BRUCELLOS
[3]   ISOLATION, IDENTIFICATION, AND CHARACTERIZATION IN ISRAEL OF BRUCELLA-MELITENSIS BIOVAR-1 ATYPICAL STRAINS SUSCEPTIBLE TO DYES AND PENICILLIN, INDICATING THE EVOLUTION OF A NEW VARIANT [J].
BANAI, M ;
MAYER, I ;
COHEN, A .
JOURNAL OF CLINICAL MICROBIOLOGY, 1990, 28 (05) :1057-1059
[4]   IDENTIFICATION OF 7 SURFACE-EXPOSED BRUCELLA OUTER-MEMBRANE PROTEINS BY USE OF MONOCLONAL-ANTIBODIES - IMMUNOGOLD LABELING FOR ELECTRON-MICROSCOPY AND ENZYME-LINKED-IMMUNOSORBENT-ASSAY [J].
CLOECKAERT, A ;
DEWERGIFOSSE, P ;
DUBRAY, G ;
LIMET, JN .
INFECTION AND IMMUNITY, 1990, 58 (12) :3980-3987
[5]  
Corbel MJ, 1984, BERGEYS MANUAL SYSTE, P377
[6]   RIBOSOMAL RIBONUCLEIC-ACID CISTRON SIMILARITIES AND TAXONOMIC NEIGHBORHOOD OF BRUCELLA AND CDC GROUP-VD [J].
DELEY, J ;
MANNHEIM, W ;
SEGERS, P ;
LIEVENS, A ;
DENIJN, M ;
VANHOUCKE, M ;
GILLIS, M .
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1987, 37 (01) :35-42
[7]   CLONING AND NUCLEOTIDE-SEQUENCE OF THE GENE CODING FOR THE MAJOR 25-KILODALTON OUTER-MEMBRANE PROTEIN OF BRUCELLA-ABORTUS [J].
DEWERGIFOSSE, P ;
LINTERMANS, P ;
LIMET, JN ;
CLOECKAERT, A .
JOURNAL OF BACTERIOLOGY, 1995, 177 (07) :1911-1914
[8]   PORINS OF BRUCELLA SPECIES [J].
DOUGLAS, JT ;
ROSENBERG, EY ;
NIKAIDO, H ;
VERSTREATE, DR ;
WINTER, AJ .
INFECTION AND IMMUNITY, 1984, 44 (01) :16-21
[9]  
DUBRAY G, 1983, ANN RECH VET, V14, P311
[10]   AMPLIFICATION FRAGMENT LENGTH POLYMORPHISM IN BRUCELLA STRAINS BY USE OF POLYMERASE CHAIN-REACTION WITH ARBITRARY PRIMERS [J].
FEKETE, A ;
BANTLE, JA ;
HALLING, SM ;
STICH, RW .
JOURNAL OF BACTERIOLOGY, 1992, 174 (23) :7778-7783