ASYMMETRY AND TRANSPOSITION RATES OF PHOSPHATIDYLCHOLINE IN RAT ERYTHROCYTE-GHOSTS

被引:171
作者
BLOJ, B
ZILVERSMIT, DB
机构
[1] CORNELL UNIV, DIV BIOL SCI, SECT BIOCHEM MOLEC & CELL BIOL, ITHACA, NY 14853 USA
[2] CORNELL UNIV, DIV NUTR SCI, 202 SAVAGE HALL, ITHACA, NY 14853 USA
关键词
D O I
10.1021/bi00651a017
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Purified phospholipid exchange protein from beef heart cytosol was used to accelerate the exchange of phospholipids between labeled sealed ghosts and phosphatidylcholine/cholesterol liposomes. The purified protein accelerates the transfer of phosphatidylcholine and, to a lesser degree, that of sphingomyelin, phosphatidylinositol and lysophosphatidylcholine. The presence of exchange protein did not accelerate the exchange of phospholipids between intact red blood cells and liposomes, but 75% of the phosphatidylcholine of sealed ghosts was readily available for exchange. The remaining 25% was also exchangeable but at a slower rate. When the exchange was assayed between insideout vesicles and liposomes, 37% of the phosphatidylcholine was readily available, and 64% was exchanged at a slower rate. These results are consistent with an asymmetric distribution of phosphatidylcholine in isolated erythrocyte membrane fractions. The sum of the forward and backward transposition of phosphatidylcholine between the inside and outside layers of sealed ghost membranes amounted to 11%/h, and the half-time for equilibration was 2.3 h. Significantly lower values were obtained for the inside-out vesicles (half-time for equilibration: 5.3 h). During the formation of the vesicles, the asymmetry of phosphatidylcloline is apparently preserved, but structural changes occur in the membrane that affect the rate of membrane transposition of phosphatidylcholine.
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页码:1277 / 1283
页数:7
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