PHOTOAFFINITY-LABELING OF DIFFERENT BENZODIAZEPINE RECEPTORS AT PHYSIOLOGICAL TEMPERATURE

被引：15

作者：

EICHINGER, A ^{[1
]}

SIEGHART, W ^{[1
]}

机构：

[1] VIENNA UNIV, PSYCHIAT KLIN, DEPT BIOCHEM PSYCHIAT, A-1090 VIENNA, AUSTRIA

来源：

JOURNAL OF NEUROCHEMISTRY | 1984年 / 43卷 / 06期

关键词：

D O I：

10.1111/j.1471-4159.1984.tb06103.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Irreversible labeling of benzodiazepine receptors in membranes from [rat] cerebellum or hippocampus was compared at O.degree. C or 37.degree. C using [3H]flunitrazepam as a photoaffinity ligand. [3H]Flunitrazepam reproducibly and irreversibly labeled mainly one protein (P51) in cerebellum and at least two proteins (P51 and P55) in hippocampus at both temperatures. Differential inhibition at 37.degree. C of irreversible [3H]flunitrazepam binding to the individual proteins by several selective benzodiazepine receptor ligands supports the hypothesis that P51 and P55 are associated with different benzodiazepine receptors.

引用

页码：1745 / 1748

页数：4

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