FATTY-ACID ALTERATION BY A DELTA-9 DESATURASE IN TRANSGENIC TOBACCO TISSUE

被引：29

作者：

GRAYBURN, WS ^{[1
]}

COLLINS, GB ^{[1
]}

HILDEBRAND, DF ^{[1
]}

机构：

[1] UNIV KENTUCKY,DEPT AGRON,LEXINGTON,KY 40546

来源：

BIO-TECHNOLOGY | 1992年 / 10卷 / 06期

关键词：

D O I：

10.1038/nbt0692-675

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Nicotiana tabacum tissue was transformed with a rat stearyl-CoA desaturase gene. Gas chromatographic analysis showed an increase in monounsaturated 16 and 18 carbon fatty acids in selected transformed calli and leaves. Fractionation of lipid classes indicated that palmitoleic acid was found in the phosphatidylcholine fraction of desaturase-transformed leaves, but not in leaves transformed with vector sequences. Plant transformation was verified by polymerase chain reaction (PCR) amplification of total leaf DNA.

引用

收藏

页码：675 / 678

页数：4

相关论文

共 25 条

[1] A MUTANT OF ARABIDOPSIS DEFICIENT IN C18-3 AND C16-3 LEAF LIPIDS [J].

BROWSE, J ;

MCCOURT, P ;

SOMERVILLE, C .

PLANT PHYSIOLOGY, 1986, 81 (03) :859-864

[2] A RAPID SCREENING TECHNIQUE FOR DETERMINING THE LIPID-COMPOSITION OF SOYBEAN SEEDS [J].

DAHMER, ML ;

FLEMING, PD ;

COLLINS, GB ;

HILDEBRAND, DF .

JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 1989, 66 (04) :543-548

[3] BROAD HOST RANGE DNA CLONING SYSTEM FOR GRAM-NEGATIVE BACTERIA - CONSTRUCTION OF A GENE BANK OF RHIZOBIUM-MELILOTI [J].

DITTA, G ;

STANFIELD, S ;

CORBIN, D ;

HELINSKI, DR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1980, 77 (12) :7347-7351

[4]

ENOCH HG, 1976, J BIOL CHEM, V251, P5095

[5] PHOSPHATIDYLCHOLINE AND ITS RELATIONSHIP TO TRIACYLGLYCEROL BIOSYNTHESIS IN OIL-TISSUES [J].

GRIFFITHS, G ;

STYMNE, S ;

STOBART, AK .

PHYTOCHEMISTRY, 1988, 27 (07) :2089-2093

[6] FATTY-ACID METABOLISM [J].

HARWOOD, JL .

ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1988, 39 :101-138

[7] A BINARY PLANT VECTOR STRATEGY BASED ON SEPARATION OF VIR-REGION AND T-REGION OF THE AGROBACTERIUM-TUMEFACIENS TI-PLASMID [J].

HOEKEMA, A ;

HIRSCH, PR ;

HOOYKAAS, PJJ ;

SCHILPEROORT, RA .

NATURE, 1983, 303 (5913) :179-180

[8] A SIMPLE AND GENERAL-METHOD FOR TRANSFERRING GENES INTO PLANTS [J].

HORSCH, RB ;

FRY, JE ;

HOFFMANN, NL ;

EICHHOLTZ, D ;

ROGERS, SG ;

FRALEY, RT .

SCIENCE, 1985, 227 (4691) :1229-1231

[9]

Kates M., 1986, TECHNIQUES LIPIDOLOG

[10]

LEE SY, 1990, BIOTECHNIQUES, V9, P676