ANTIGENIC CROSS-REACTIVITY OF VENOMS FROM MEDICALLY IMPORTANT LOXOSCELES (ARANEAE) SPECIES IN BRAZIL

被引：40

作者：

BARBARO, KC ^{[1
]}

EICKSTEDT, VRD ^{[1
]}

MOTA, I ^{[1
]}

机构：

[1] INST BUTANTAN,ARTROPODOS PECONHENTOS LAB,BR-05503900 SAO PAULO,SP,BRAZIL

来源：

TOXICON | 1994年 / 32卷 / 01期

基金：

巴西圣保罗研究基金会;

关键词：

D O I：

10.1016/0041-0101(94)90027-2

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Antigenic cross-reactivity between the components of venoms from three spiders of the genus Loxosceles, L. gaucho, L. laeta and L. intermedia, was studied. Species-specific antisera were prepared by immunization of rabbits with each venom. Anti-L. gaucho horse hyperimmune serum provided by the Butantan Institute for treatment of accidents with these spiders was also used. Separation by SDS-PAGE showed the existence of many common components in the three antigens. No individual antigen was observed. Analysis of the antisera by ELISA and Western blotting showed cross-reactivity as well as several common bands between the three venoms. The horse anti-L. gaucho venom serum recognized many common proteins when antigens of the other two species were used. Antigens in the range of 33,000-35,000 mol, wt showed most crossreactivity. Both horse and rabbit anti-venom sera contained antibodies able to neutralize the lethal and dermonecrotic activities of the venom of the three species studied.

引用

页码：113 / 120

页数：8

共 15 条

[1] PURIFICATION AND CHARACTERIZATION OF A TOXIN FROM BROWN RECLUSE SPIDER (LOXOSCELES-RECLUSA) VENOM GLAND EXTRACTS [J].

BABCOCK, JL ;

CIVELLO, DJ ;

GEREN, CR .

TOXICON, 1981, 19 (05) :677-689

[2] DERMONECROTIC AND LETHAL COMPONENTS OF LOXOSCELES-GAUCHO SPIDER VENOM [J].

BARBARO, KC ;

CARDOSO, JLC ;

EICKSTEDT, VRD ;

MOTA, I .

TOXICON, 1992, 30 (03) :331-338

[3]

Cardoso J.L.C., 1988, REV SOC BRAS TOXICOL, V1, P58

[4]

CARDOSO JLC, 1990, MEM I BUTANTAN, V52, P81

[5]

FURLANETTO RS, 1962, CIENCIA CULTURA, V14, P254

[6] LOXOSCELISM [J].

FUTRELL, JM .

AMERICAN JOURNAL OF THE MEDICAL SCIENCES, 1992, 304 (04) :261-267

[7] ISOLATION AND CHARACTERIZATION OF TOXINS FROM BROWN RECLUSE SPIDER VENOM (LOXOSCELES-RECLUSA) [J].

GEREN, CR ;

CHAN, TK ;

HOWELL, DE ;

ODELL, GV .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1976, 174 (01) :90-99

[8]

Gertsch W. J., 1983, B AM MUS NAT HIST, V175, P264

[9] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[10]

LOWRY OH, 1951, J BIOL CHEM, V193, P265

← 1 2 →