SEQUENCE OF THE PEH GENE OF ERWINIA-CAROTOVORA - HOMOLOGY BETWEEN ERWINIA AND PLANT ENZYMES

被引：50

作者：

HINTON, JCD

GILL, DR

LALO, D

PLASTOW, GS

SALMOND, GPC

机构：

[1] UNIV WARWICK, DEPT BIOL SCI, COVENTRY CV4 7AL, W MIDLANDS, ENGLAND

[2] UNIV LEICESTER, LEICESTER BIOCTR, LEICESTER LE1 7RH, ENGLAND

[3] DALGETY PLC, GRP RES LAB, CAMBRIDGE CB1 2JN, ENGLAND

来源：

MOLECULAR MICROBIOLOGY | 1990年 / 4卷 / 06期

关键词：

D O I：

10.1111/j.1365-2958.1990.tb00675.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Polygalacturonase (Peh) and other pectolytic enzymes play a crucial role in the maceration of vegetables by soft rot Erwinia spp. We have sequenced the peh gene of Erwinia carotovora subsp. carotovora, and identified its product as a precursor of molecular weight 42639, and a mature protein of molecular weight 42200. A putative KdgR‐binding site was identified in the region 5’to the peh gene. The Peh protein showed significant homology with Peh from tomato. In addition, we have found homologies between pectin methylesterase and pectate lyase from Erwinia and their counterparts in tomato. These homologies are described, and their significance discussed. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：1029 / 1036

页数：8

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