A PROTEIN OF NEUTROPHIL GRANULES INTERFERES WITH ACTIVATION OF NADPH OXIDASE IN A CELL-FREE SYSTEM

被引：6

作者：

AVIRAM, I

FABER, A

机构：

[1] The Department of Biochemistry, Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 169卷 / 01期

关键词：

D O I：

10.1016/0006-291X(90)91454-Z

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A soluble extract of neutrophil granules interfered with activation of the NADPH oxidase in a cell-free system. The extract had no effect on superoxide production by preactivated enzyme. The inhibitory activity was retained during dialysis and was lost upon exposure to proteinase K indicating that the active substance was a protein. The inhibitor exhibited a high stability at elevated temperatures. Chromatography of granules extract on ion exchangers implied that the inhibitor was a positively charged protein eluting from S Sepharose cation exchanger above 0.4M concentration of NaCl. © 1990.

引用

页码：198 / 202

页数：5

共 15 条

[1]

AKARD LP, 1988, BLOOD, V72, P322

[2] KINETICS OF CELL-FREE ACTIVATION OF NEUTROPHIL NADPH OXIDASE - EFFECTS OF NEOMYCIN AND GUANINE-NUCLEOTIDES [J].