SACCHAROMYCES-CEREVISIAE STRAINS THAT OVEREXPRESS HETEROLOGOUS PROTEINS

被引：62

作者：

SLEEP, D

BELFIELD, GP

BALLANCE, DJ

STEVEN, J

JONES, S

EVANS, LR

MOIR, PD

GOODEY, AR

机构：

[1] Delta Biotechnology Limited, Nottingham NG7 1FD, Castle Court

来源：

BIO-TECHNOLOGY | 1991年 / 9卷 / 02期

关键词：

D O I：

10.1038/nbt0291-183

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We describe a system that facilitates the selection of host mutants that overproduce a range of secreted and internally produced heterologous proteins in Saccharomyces cerevisiae. These mutants were initially selected for their ability to oversecrete recombinant human albumin (rHA), as detected by a direct visual assay that relies upon antibody precipitation in solid media. Yeast strains that were able to synthesize and secrete increased levels of rHA also produced elevated levels of internally expressed proteins including alpha-1-antitrypsin Pittsburgh variant and plasminogen activator inhibitor type 2.

引用

页码：183 / 187

页数：5

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