ISOLATION OF A HUMAN-CHROMOSOME 14-ONLY SOMATIC-CELL HYBRID - ANALYSIS USING ALU AND LINE-BASED PCR

被引：11

作者：

MARES, A

LEDBETTER, SA

LEDBETTER, DH

ROBERTS, R

HEJTMANCIK, JF

机构：

[1] BAYLOR COLL MED,INST MOLEC GENET,DEPT MED,HOUSTON,TX 77030

[2] NEI,BETHESDA,MD 20892

来源：

GENOMICS | 1991年 / 11卷 / 01期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0888-7543(91)90122-U

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Interspecific somatic cell hybrids containing single human chromosomes are valuable reagents for localization of cloned genes and DNA fragments to specific chromosomes, for the development of chromosome-specific libraries, and for generation of hybrid cell lines containing subchromosomal regions. A CHO somatic cell hybrid containing a single, intact human chromosome 14 (MHR14) was developed and confirmed by LINE PCR amplification gel pattern, by Alu-517 PCR product dot blot hybridization, and by cytogenetic analysis. MHR14 will serve as the chromosome source for the development of a radiation map of human chromosome 14. © 1991.

引用

页码：215 / 218

页数：4

共 9 条

[1] SIMULTANEOUS IDENTIFICATION OF CHROMATID REPLICATION AND OF HUMAN-CHROMOSOMES IN METAPHASES OF MAN-MOUSE SOMATIC-CELL HYBRIDS [J].