A NEW PRODUCTION METHOD FOR GLUCOSE-OXIDASE

被引：10

作者：

DEBAETSELIER, A

DOHET, P

DEBEUKELAER, M

HATHI, V

HANOTIER, J

FREDERICK, K

ROSENBERG, S

VASAVADA, A

机构：

[1] ZONING IND C, FINA RES, B-6520 FELUY, BELGIUM

[2] CHIRON CORP, CHIRON RES LABS, EMERYVILLE, CA 94608 USA

来源：

JOURNAL OF BIOTECHNOLOGY | 1992年 / 24卷 / 02期

关键词：

SACCHAROMYCES-CEREVISIAE; RECOMBINANT GLUCOSE OXIDASE; SECRETION; PURIFICATION;

D O I：

10.1016/0168-1656(92)90117-R

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The recombinant yeast expression plasmid, pSGO2, has been constructed containing a hybrid yeast alcohol dehydrogenase II-glyceraldehyde-3-phosphate dehydrogenase promoter, the glucose oxidase presequence and the mature glucose oxidase coding sequence. When transformed into yeast, this plasmid directs the synthesis and secretion of more than 1.5 g l-1 of active glucose oxidase. Analysis of the yeast-derived enzyme shows extensive N-linked glycosylation in comparison to the A. niger protein. Both proteins show similar specific activities in terms of U mg-1 of protein.

引用

页码：141 / 148

页数：8

共 12 条

[1]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[2]

DEBAETSELIER QA, 1991, BIO-TECHNOL, V7, P559

[3]

FREDERICK KR, 1990, J BIOL CHEM, V265, P3793

[4] TRANSFORMATION OF YEAST [J].