THE MAJOR RNA IN PROSOMES OF HELA-CELLS AND DUCK ERYTHROBLASTS IS TRANSFER RNA(LYS,3)

被引:37
作者
NOTHWANG, HG
COUX, O
KEITH, G
SILVAPEREIRA, I
SCHERRER, K
机构
[1] UNIV PARIS 07,CNRS,INST JACQUES MONOD,2 PL JUSSIEU,F-75251 PARIS 05,FRANCE
[2] UNIV STRASBOURG 1,CNRS,INST BIOL MOLEC & CELLULAIRE,F-67084 STRASBOURG,FRANCE
[3] UNIV STRASBOURG 1,CTR RECH BIOL MOLEC & CELLULAIRE,F-67084 STRASBOURG,FRANCE
关键词
D O I
10.1093/nar/20.8.1959
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Two-dimensional gel electrophoresis of HeLa cell prosomal RNAs, 3'-end labeled by RNA ligase, revealed one prominent spot. Determination of a partial sequence at the 3'-end indicated full homology to the 18 nucleotides at the 3'-end of tRNA(Lys,3) from rabbit, the bovine and the human species. An oligonucleotide complementary to the 3'-end of tRNA(Lys,3) hybridized on Northern blots with prosomal RNA from both HeLa cells and duck erythroblasts. In two-dimensional PA-GE, the major pRNA of HeLa cells co-migrated with bovine tRNA(Lys,3). Reconstitution of the CCA 3'-end of RNA from both human and duck prosomes, by tRNA-nucleotidyl-transferase, confirmed the tRNA character of this type of RNA. Furthermore, it revealed at least one additional tRNA band about 85 nt long among the prosomal RNA from both species. Finally, confirming an original property of prosomal RNA, we show that in vitro synthesized tRNA(lys,3) hybridizes stably to duck globin mRNA, and to poly(A)+- and poly(A)--RNA from HeLa cells.
引用
收藏
页码:1959 / 1965
页数:7
相关论文
共 31 条
[1]   THE GLUCOCORTICOID RECEPTOR PROTEIN BINDS TO TRANSFER-RNA [J].
ALI, M ;
VEDECKIS, WV .
SCIENCE, 1987, 235 (4787) :467-470
[2]   CODON USAGE TABULATED FROM THE GENBANK GENETIC SEQUENCE DATA [J].
AOTA, S ;
GOJOBORI, T ;
ISHIBASHI, F ;
MARUYAMA, T ;
IKEMURA, T .
NUCLEIC ACIDS RESEARCH, 1988, 16 :R315-R402
[3]   IDENTITY OF THE 19S PROSOME PARTICLE WITH THE LARGE MULTIFUNCTIONAL PROTEASE COMPLEX OF MAMMALIAN-CELLS (THE PROTEASOME) [J].
ARRIGO, AP ;
TANAKA, K ;
GOLDBERG, AL ;
WELCH, WJ .
NATURE, 1988, 331 (6152) :192-194
[4]   CHARACTERIZATION OF THE PROSOME FROM DROSOPHILA AND ITS SIMILARITY TO THE CYTOPLASMIC STRUCTURES FORMED BY THE LOW-MOLECULAR WEIGHT HEAT-SHOCK PROTEINS [J].
ARRIGO, AP ;
DARLIX, JL ;
KHANDJIAN, EW ;
SIMON, M ;
SPAHR, PF .
EMBO JOURNAL, 1985, 4 (02) :399-406
[5]   HIV-1 REVERSE-TRANSCRIPTASE SPECIFICALLY INTERACTS WITH THE ANTICODON DOMAIN OF ITS COGNATE PRIMER TRANSFER-RNA [J].
BARAT, C ;
LULLIEN, V ;
SCHATZ, O ;
KEITH, G ;
NUGEYRE, MT ;
GRUNINGERLEITCH, F ;
BARRESINOUSSI, F ;
LEGRICE, SFJ ;
DARLIX, JL .
EMBO JOURNAL, 1989, 8 (11) :3279-3285
[6]   STRUCTURAL AND SEROLOGICAL SIMILARITY OF MHC-LINKED LMP AND PROTEASOME (MULTICATALYTIC PROTEINASE) COMPLEXES [J].
BROWN, MG ;
DRISCOLL, J ;
MONACO, JJ .
NATURE, 1991, 353 (6342) :355-357
[7]   EUKARYOTIC PRE-TRANSFER RNA 5' PROCESSING NUCLEASE - COPURIFICATION WITH A COMPLEX CYLINDRICAL PARTICLE [J].
CASTANO, JG ;
ORNBERG, R ;
KOSTER, JG ;
TOBIAN, JA ;
ZASLOFF, M .
CELL, 1986, 46 (03) :377-387
[9]  
DAHLMANN B, 1988, BIOCHEM J, V255, P750
[10]   PROSOMES - UBIQUITY AND INTERSPECIES STRUCTURAL VARIATION [J].
DESA, CM ;
DESA, MFG ;
AKHAYAT, O ;
BRODERS, F ;
SCHERRER, K ;
HORSCH, A ;
SCHMID, HP .
JOURNAL OF MOLECULAR BIOLOGY, 1986, 187 (04) :479-493