KINETIC CHARACTERIZATION OF THE EFFECTS OF ORGANIC-SOLVENTS ON THE PERFORMANCE OF A PEROXIDASE-MODIFIED ELECTRODE IN DETECTING PEROXIDES, THIOUREA AND ETHYLENETHIOUREA

被引：27

作者：

ADEYOJU, O

IWUOHA, EI

SMYTH, MR

机构：

[1] School of Chemical Sciences, Dublin City University, Dublin

来源：

ELECTROANALYSIS | 1995年 / 7卷 / 10期

关键词：

PEROXIDASE; INHIBITION; ORGANIC PHASE BIOSENSOR; HORSERADISH PEROXIDASE;

D O I：

10.1002/elan.1140071003

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Horseradish peroxidase (HRP) modified electrodes were constructed by entrapping the enzyme in an Eastman AQ 55D polymer. The biosensor detects butanone peroxide (BTP) as a substrate, and thiourea (THU) and ethylenethiourea (ETU) as HRP inhibitors. The kinetic parameters for this sensor in organic media such as I-max and K-m' for BTP detection and K-i' for ETU and THU detection were evaluated. The differences in the values of these parameters were explained in terms of the physico-chemical properties of the organic reaction media. A possible solvent-induced deviation of the biosensor behavior from Michaelis-Menten kinetics in the detection of ETU and THU was evaluated using the Hill analysis. The least deviation was observed in acetonitrile. Spectroelectrochemistry was also used to qualitatively assess solvent-HRP, BTP-HRP and inhibitor-HRP interactions.

引用

页码：924 / 929

页数：6

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