ISOLATION OF 9 HUMAN PLASMA PROTEINASE-INHIBITORS BY SEQUENTIAL AFFINITY-CHROMATOGRAPHY

被引：16

作者：

DUBIN, A

POTEMPA, J

TRAVIS, J

机构：

[1] JAGIELLONIAN UNIV,INST MOLEC BIOL,PL-31120 KRAKOW,POLAND

[2] UNIV GEORGIA,DEPT BIOCHEM,ATHENS,GA 30602

来源：

PREPARATIVE BIOCHEMISTRY | 1990年 / 20卷 / 01期

关键词：

D O I：

10.1080/00327489008050177

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Purification of nine plasma proteinase inhibitors and one zymogen from a single batch of human plasma, using affinity chromatography has been accomplished. Those isolated were plasminogen (lysine-Sepharose), alpha-2-antiplasmin (plasminogen-Sepharose), high and low molecular weight kini^gens (CM-papain-Sepharose), alpha-2-macroglobulin (Zn chelate-Sepharose), alpha-1-proteinase inhibitor, alpha-l-antichymotrypsin, Cl-inhibitor, inter-alpha-trypsin inhibitor (Blue-Sepharose) and antithrombin III (heparin-Sepharose). Alpha-2-macroglobulin and alpha-l-proteinase inhibitor required gel filtration as additional purification steps. Each protein was recovered in both high yield and purity. © 1990, Taylor & Francis Group, LLC. All rights reserved.

引用

页码：63 / 74

页数：12

共 21 条

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