CHEMILUMINESCENCE SANDWICH ENZYME-IMMUNOASSAY FOR DETERMINATION OF HUMAN GRANULOCYTE-COLONY-STIMULATING FACTOR (G-CSF)

A chemiluminescence sandwich enzyme immunoassay, using a glucose oxidase (GO) label. was developed for detecting attomole amounts of human granulocyte colony stimulating factor (G-CSF), Purified goat F(ab')(2) immobilized on a bead and purified goat Fab' labelled with GO were selected in combination with a chemiluminescent detection system comprising luminol and ferricyanide, The detection limits for G-CSF were 4 amol/assay (1 pg/mL) in buffer solution and 10 amol/assay (2.5 pg/mL) in human serum, Coefficients of variation within assay and between assay ranged from 5.5% to 7.8% and from 3.4% to 16.0%, respectively, The G-CSF content of serum from normal healthy individuals was measurable using this method, G-CSF in 24 normal human sera showed a mean value of 19.3 pg/mL and ranged from 3.6 to 83.0 pg/mL.