IDENTIFICATION OF THE LARGE SUBUNIT OF RIBULOSE 1,5-BISPHOSPHATE CARBOXYLASE OXYGENASE AS A SUBSTRATE FOR TRANSGLUTAMINASE IN MEDICAGO-SATIVA L (ALFALFA)

被引：79

作者：

MARGOSIAK, SA ^{[1
]}

DHARMA, A ^{[1
]}

BRUCECARVER, MR ^{[1
]}

GONZALES, AP ^{[1
]}

LOUIE, D ^{[1
]}

KUEHN, GD ^{[1
]}

机构：

[1] NEW MEXICO STATE UNIV,DEPT CHEM,PLANT GENET ENGN LAB,LAS CRUCES,NM 88003

来源：

PLANT PHYSIOLOGY | 1990年 / 92卷 / 01期

关键词：

D O I：

10.1104/pp.92.1.88

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Extracts prepared from floral meristematic tissue of alfalfa (Medicago sativa L.) were investigated for expression of the enzyme transglutaminase in order to identify the major protein substrate for transglutaminase-directed modifications among plant proteins. The large polymorphic subunits of ribulose 1,5-bisphosphate carboxylase/oxygenase in alfalfa, with molecular weights of 52,700 and 57,600, are major substrates for transglutaminase in these extracts. This was established by: (a) covalent conjugation of monodansylcadaverine to the large subunit followed by fluorescent detection in SDS-polyacrylamide gels; (b) covalent conjugation of [14C]putrescine to the large subunit with detection by autoradiography; (c) covalent conjugation of monodansylcadaverine to the large subunit and demonstration of immunocross-reactivity on nitrocellulose transblot of the modified large subunit with antibody prepared in rabbits against dansylated-ovalbumin; (d) demonstration of a direct dependence of the rate of transglutaminase-mediated, [14C]putrescine incorporation upon the concentration of ribulose, 1,5-bisphosphate carboxylase/oxygenase from alfalfa or spinach; and (e) presumptive evidence from size exclusion chromatography that transglutaminase may cofractionate with native molecules of ribulose 1,5-bisphosphate carboxylase/oxygenase in crude extracts. Analysis of the primary structure of plant large subunit has revealed numerous potential glutaminyl and lysyl sites for transglutaminase-directed modifications of ribulose 1,5-bisphosphate carboxylase/oxygenase.

引用

页码：88 / 96

页数：9

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