K+-EVOKED DOPAMINE RELEASE DEPENDS ON A CYTOSOLIC CA2+ POOL REGULATED BY N-TYPE CA2+ CHANNELS

被引：16

作者：

DEERAUSQUIN, G

BROOKER, G

HANBAUER, I

机构：

[1] NHLBI,CHEM PHARMACOL LAB,BLDG 10,RM 8N-102,BETHESDA,MD 20892

[2] GEORGETOWN UNIV,FIDIA GEORGETOWN INST NEUROSCI,WASHINGTON,DC 20007

来源：

NEUROSCIENCE LETTERS | 1992年 / 145卷 / 02期

关键词：

PRIMARY CULTURE; MESENCEPHALON; DOPAMINE RELEASE; CA2+ CHANNEL; SPATIAL SIGNAL; FURA-2; FLUORESCENCE; SINGLE-CELL CALCIUM IMAGING;

D O I：

10.1016/0304-3940(92)90001-N

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Membrane depolarization evoked by 25-40 mM K+ elicited an immediate increase of somatic and neuritic [Ca2+]i in cultured dopaminergic neurons as measured by digital fluorescence microscope imaging. The rise of neuritic [Ca2+]i was inhibited by N-type but not L-type Ca2+ channel blockers, while the rise of somatic [Ca2+]i was prevented by both L- and N-type Ca2+ channel blockers. Similarly, depolarization-induced [H-3]dopamine release was selectively attenuated by N-type Ca2+ channel blockers. The present results suggest that [H-3]dopamine release from mesencephalic neuronal cell cultures relates to a Ca2+-dependent mechanism regulated by N-type channels located in the vicinity of the exocytotic sites within neuritic processes.

引用

页码：121 / 125

页数：5

共 15 条

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