PRODUCTION OF HIGH-CONTENT FRUCTO-OLIGOSACCHARIDES BY AN ENZYMATIC SYSTEM FROM PENICILLIUM-RUGULOSUM

The production of high-content fructo-oligosaccharides from sucrose by a crude FTF from a new strain of Penicillium isolated in our Laboratory was investigated. The optimum conditions for the production of the enzyme acid for the enzymic reaction have been determined. It has been demonstrated that the crude enzyme acts as a mixed enzyme system of fructosyl transferase (FTF; Class 2 of Enzyme Nomenclature) and glycosidases (Class 3 of Enyme Nomenclature), Under optimum conditions: pH 5.5, temperature 55 degrees C, sucrose concentration 750 g/l, enzyme concentration 5 FTF units/g sucrose; conversion yield up to 80%, were obtained and high concentration of nystose (412 g/l) and fructofuranosyl-nystose (176 g/l) were accumulated.