TEMPERATURE-DEPENDENCE OF CHLOROPHYLL FLUORESCENCE FROM THE LIGHT-HARVESTING COMPLEX II OF HIGHER-PLANTS

被引:44
作者
RUBAN, AV [1 ]
DEKKER, JP [1 ]
HORTON, P [1 ]
VANGRONDELLE, R [1 ]
机构
[1] UNIV SHEFFIELD, ROBERT HILL INST, DEPT MOLEC BIOL & BIOTECHNOL, SHEFFIELD S10 2UH, S YORKSHIRE, ENGLAND
关键词
D O I
10.1111/j.1751-1097.1995.tb03964.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Chlorophyll fluorescence spectra of LCHII, the light harvesting complex of photosystem II, have been recorded in the aggregated and trimeric forms for a range of temperatures from 293 to 4 K. At least five long-wavelength emitters in the 682-702 nm region with different temperature dependencies were found in the spectra of the aggregates. At 293 K the yield of LCHII trimers was higher than aggregates by a factor of 4, but, upon lowering the temperature, a fluorescence rise was observed which was much stronger for LCHII aggregates than for LCHII trimers, so that at 4 K their yields were comparable. The implications of these data in terms of the function of LCHII are discussed.
引用
收藏
页码:216 / 221
页数:6
相关论文
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[21]   FLUORESCENCE POLARIZATION AND LOW-TEMPERATURE ABSORPTION-SPECTROSCOPY OF A SUBUNIT FORM OF LIGHT-HARVESTING COMPLEX-I FROM PURPLE PHOTOSYNTHETIC BACTERIA [J].
VISSCHERS, RW ;
CHANG, MC ;
VANMOURIK, F ;
PARKESLOACH, PS ;
HELLER, BA ;
LOACH, PA ;
VANGRONDELLE, R .
BIOCHEMISTRY, 1991, 30 (23) :5734-5742