INTERLEUKIN-1 AND TUMOR-NECROSIS-FACTOR STIMULATE CGMP FORMATION IN RAT RENAL MESANGIAL CELLS

Treatment of mesangial cells with recombinant human interleukin 1β (IL-1β) or recombinant human tumor necrosis factor α (TNFα) dose-dependently increased cGMP formation. Both IL-1β and TNFα-stimulated formation of cGMP occurred after a initial lag period of 4 to 8 hours. Treatment of cells with actinomycin D, cycloheximide or dexamethason completely abolished cytokine-induced cGMP formation. Furthermore, the guanylate cyclase inhibitor Methylene blue completely blocked IL-1β- and TNFα-stimulated cGMP generation. NG-mono-methyl-L-arginine attenuated IL-1β- and TNFα-induced cGMP production, an effect that was reversed by L-arginine. © 1990.