DNA TOPOISOMERASE-I FROM ALCALIGENES-EUTROPHUS H16

被引：3

作者：

ANDERA, L ^{[1
]}

MIKULIK, K ^{[1
]}

机构：

[1] CZECHOSLOVAK ACAD SCI, INST MICROBIOL, CS-14220 PRAGUE, CZECHOSLOVAKIA

来源：

ARCHIVES OF MICROBIOLOGY | 1990年 / 153卷 / 02期

关键词：

Alcaligenes eutrophus; DNA topoisomerase I; Intercalation drugs; Polyamines;

D O I：

10.1007/BF00247810

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Molecular and functional properties of DNA topoisomerase I isolated from a hydrogen-oxidizing bacterium, Alcaligenes eutrophus H16, were investigated. Under native conditions the enzyme forms a monomer with a relative molar mass of 98.500. A rod-like shape of the molecule was derived from the calculated frictional coefficient. The isoelectric point of the enzyme was determined to be in the range of 7.6-8.0. The enzyme activity is strictly Mg2+ dependent with an optimum at 3 mM Mg2+. The pH optimum ranges within 7.5-9.0. A. eutrophus DNA topoisomerase I activity is inhibited by M13 ssDNA, high ionic strength, polyamines, heparin and by a number of intercalating drugs. © 1990 Springer-Verlag.

引用

页码：134 / 138

页数：5

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