BIOLOGICAL PRODUCTION OF SEMISYNTHETIC OPIATES USING GENETICALLY-ENGINEERED BACTERIA

被引：24

作者：

FRENCH, CE ^{[1
]}

HAILES, AM ^{[1
]}

RATHBONE, DA ^{[1
]}

LONG, MT ^{[1
]}

WILLEY, DL ^{[1
]}

BRUCE, NC ^{[1
]}

机构：

[1] UNIV CAMBRIDGE,INST BIOTECHNOL,CAMBRIDGE CB2 1QT,ENGLAND

来源：

BIO-TECHNOLOGY | 1995年 / 13卷 / 07期

关键词：

D O I：

10.1038/nbt0795-674

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Semisynthetic derivatives of morphine and related alkaloids re in widespread clinical use. Due to the complexity of these molecules, however, chemical transformations are difficult to achieve in high yields. We recently identified the powerful analgesic hydromorphone as an intermediate in the metabolism of morphine by Pseudomonas putida M10. Here we describe the construction of recombinant strains of Escherichia coli that express morphine dehydrogenase and morphinone reductase. These strains are capable of efficiently transforming the naturally occurring alkaloids morphine and codeine to hydro-morphone and antitussive hydrocodone, respectively. Our results demonstrate the potential for recombinant DNA technology to provide biological routes for the synthesis of known and novel semi-synthetic opiate drugs.

引用

页码：674 / 676

页数：3

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