POLYMERASE CHAIN-REACTION FOR DIAGNOSIS OF PORCINE PROLIFERATIVE ENTEROPATHY

被引：44

作者：

MCORIST, S ^{[1
]}

GEBHART, CJ ^{[1
]}

LAWSON, GHK ^{[1
]}

机构：

[1] UNIV MINNESOTA,COLL VET MED,DEPT VET PATHOBIOL,ST PAUL,MN

来源：

VETERINARY MICROBIOLOGY | 1994年 / 41卷 / 03期

基金：

英国惠康基金;

关键词：

PIG; PROLIFERATIVE ENTEROPATHY; DIAGNOSIS; BACTERIA GENERAL; PCR;

D O I：

10.1016/0378-1135(94)90101-5

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A polymerase chain reaction (PCR) assay for detection of the intracellular bacteria, ileal symbiont intracellularis of porcine proliferative enteropathy is described. The test is based on specific DNA primers and gave positive PCR product from samples of preserved intestinal mucosa and faeces from affected pigs. Mucosa and faeces from normal pigs gave no positive PCR products. The identity of the PCR product was confirmed by DNA-DNA hybridization with a probe, pCL078, specific for IS intracellularis. Positive results were of only observed in animals with active lesions of proliferative enteropathy. PCR is probably the most useful method for diagnosis of proliferative enteropathy that is currently available for live animals.

引用

页码：205 / 212

页数：8

共 18 条

[1] POLYMERASE CHAIN-REACTION FOR DETECTION OF ADENOVIRUSES IN STOOL SAMPLES [J].

ALLARD, A ;

GIRONES, R ;

JUTO, P ;

WADELL, G .

JOURNAL OF CLINICAL MICROBIOLOGY, 1990, 28 (12) :2659-2667

[2] SENSITIVE DETECTION OF TREPONEMA-PALLIDUM BY USING THE POLYMERASE CHAIN-REACTION [J].

BURSTAIN, JM ;

GRIMPREL, E ;

LUKEHART, SA ;

NORGARD, MV ;

RADOLF, JD .

JOURNAL OF CLINICAL MICROBIOLOGY, 1991, 29 (01) :62-69

[3] CLONED DNA PROBES SPECIFIC FOR THE INTRACELLULAR CAMPYLOBACTER-LIKE ORGANISM OF PORCINE PROLIFERATIVE ENTERITIS [J].

GEBHART, CJ ;

LIN, GF ;

MCORIST, SM ;

LAWSON, GHK ;

MURTAUGH, MP .

JOURNAL OF CLINICAL MICROBIOLOGY, 1991, 29 (05) :1011-1015

[4] SPECIES-SPECIFIC DNA PROBES FOR CAMPYLOBACTER SPECIES ISOLATED FROM PIGS WITH PROLIFERATIVE ENTERITIS [J].

GEBHART, CJ ;

MURTAUGH, MP ;

LIN, GF ;

WARD, GE .

VETERINARY MICROBIOLOGY, 1990, 24 (3-4) :367-379

[5]

GEBHART CJ, 1993, INT J SYST BACTERIOL, P43

[6] DIRECT POLYMERASE CHAIN-REACTION TEST FOR DETECTION OF HELICOBACTER-PYLORI IN HUMANS AND ANIMALS [J].

HO, SA ;

HOYLE, JA ;

LEWIS, FA ;

SECKER, AD ;

CROSS, D ;

MAPSTONE, NP ;

DIXON, MF ;

WYATT, JI ;

TOMPKINS, DS ;

TAYLOR, GR ;

QUIRKE, P .

JOURNAL OF CLINICAL MICROBIOLOGY, 1991, 29 (11) :2543-2549

[7]

HUBBARD AL, 1993, BREAST, V2, P50

[8] ENHANCED DETECTION OF INTRACELLULAR ORGANISM OF SWINE PROLIFERATIVE ENTERITIS, ILEAL SYMBIONT INTRACELLULARIS, IN FECES BY POLYMERASE CHAIN-REACTION [J].

JONES, GF ;

WARD, GE ;

MURTAUGH, MP ;

LIN, G ;

GEBHART, CJ .

JOURNAL OF CLINICAL MICROBIOLOGY, 1993, 31 (10) :2611-2615

[9] INTRACELLULAR BACTERIA OF PORCINE PROLIFERATIVE ENTEROPATHY - CULTIVATION AND MAINTENANCE INVITRO [J].

LAWSON, GHK ;

MCORIST, S ;

JASNI, S ;

MACKIE, RA .

JOURNAL OF CLINICAL MICROBIOLOGY, 1993, 31 (05) :1136-1142

[10] SEROLOGICAL DIAGNOSIS OF THE PORCINE PROLIFERATIVE ENTEROPATHIES - IMPLICATIONS FOR ETIOLOGY AND EPIDEMIOLOGY [J].

LAWSON, GHK ;

MCORIST, S ;

ROWLAND, AC ;

MCCARTNEY, E ;

ROBERTS, L .

VETERINARY RECORD, 1988, 122 (23) :554-557

← 1 2 →