DEVELOPMENTALLY REGULATED CELL-SURFACE STRUCTURES ON MOUSE AND HUMAN EMBRYONAL CARCINOMA CELL-LINES

Three monoclonal antibodies 5.1.H, 8.7.D and 13.7.A raised against semi-purified Tera 1 membrane fractions recognize distinct onco-fetal antigens which are developmentally regulated on cells such as Tera 2 clone 13 and appear to be restricted in their expression to undifferentiated ectoblastic cells and certain organized cystic structures mimicking the fetal intestine. These antigens, absent from normal adult tissues, differ markedly from glycosidic stage-specific antigens such as 75.12 which, while functioning as embryonal carcinoma differentiation markers, are also expressed on certain adult tissues. No evidence for a role of fucosyltransferases in regulating either 75.12 or SSEA-1 antigen exprssion on embryonal carcinoma cells or for the presence of lectin-like structures recognizing these antigens on such cells was found.