CYTOKINE EXPRESSION IN HUMAN ENDOMETRIUM THROUGHOUT THE MENSTRUAL-CYCLE

被引：177

作者：

TABIBZADEH, S

SUN, XZ

机构：

[1] Department of Pathology, University of South Florida Health Sciences Center, Moffitt Cancer Center, Tampa

来源：

HUMAN REPRODUCTION | 1992年 / 7卷 / 09期

关键词：

CYTOKINE; ENDOMETRIUM; GROWTH FACTOR; MESSENGER RNA; POLYMERASE CHAIN REACTION;

D O I：

10.1093/oxfordjournals.humrep.a137829

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Recent evidence suggests that diverse endometrial functions may be regulated by cytokines. In this report, the presence of protein and mRNA of cytokines were studied in human endometrium throughout the menstrual cycle. The presence of the interleukin-1 (IL-1)alpha, interleukin-1 (IL-1)beta, interleukin receptor antagonist (IRAP), interleukin-6 (IL-6) and transforming growth factor (TGF)-alpha proteins were demonstrated by immunohistochemical staining. The IL-1alpha and TGF-alpha proteins were strongly expressed and IL-1beta protein was weakly expressed in all the cells in the stroma as well as epithelial cells. IRAP was markedly expressed in die cells with morphological features of macrophages scattered in the stroma, and the expression of IL-6 protein was predominant in the endometrial epithelium. Diffuse cytoplasmic expression of IL-1alpha in endometrial epithelium during the proliferative phase contrasted markedly with its enhanced luminal expression during the secretory phase of the menstrual cycle. In addition, the presence of the mRNA of these cytokines in endometrium was established throughout the entire menstrual cycle by reverse transcription-polymerase chain reaction (RT - PCR). Abundant expression of cytokines in human endometrium emphasizes the significant roles that cytokines play in cell - cell interactions and in regulating endometrial functions.

引用

页码：1214 / 1221

页数：8

共 37 条

[1]

Arend W.P., Interleukin 1 receptor antagonist. A new member of the interleukin family, J. Din. Invest, 88, pp. 1445-1451, (1991)

[2]

Arend W.P., Welgus H.G., Thompson R.C., Eisenberg S.P., Biological properties of recombinant human monocyte-derived interleukin 1 receptor antagonist, J. Gin. Invest, 85, pp. 1694-1697, (1990)

[3]

Carter D.B., Diebel M.R., Dunn C.J., Tomich C.-S.C., Laborde A.L., Slightom J.L., Berger A.E., Bienkowski M.J., Sun F.F., McEwan R.N., Harris P., Yem A.W., Waszak G.A., Oiosay J.G., Sieu L.C., Haidee M.M., Zurher-Neety H.A., Reankm I.M., Heinrikson R.L., Truesdell S.E., Shelly J.A., Eessalu T.E., Taylor B.M., Tracey D.E., Purification, cloning, expression and biological characterization of an interleukin-1 receptor antagonist protein, Nature, 244, pp. 633-638, (1990)

[4]

Farrell R.E., Methodologies for RNA characterization. I: The isolation and characterization of mammalian RNA, Clin. Biotech, 1, pp. 50-58, (1989)

[5]

Fox H.S., Bond B.L., Parslow J.G., Estrogen regulates the IFN-7 promoter, J. Immunol, 146, pp. 4362-4367, (1991)

[6]

Hannum C.H., Wicox C.J., Arend W.P., Joslin F.G., Dripps D.J., Heimdal P.L., Armes L.G., Eisenberg S.P., Thompson R.-C., Interleukin-1 receptor antagonist activity of a human interleukin-1 inhibitor, Nature, 343, pp. 336-340, (1990)

[7]

Hsu S.M., Raine L., Fanger H., Use of avidiivbiotin-peroxidase complex (ABC) in immunoperoxidase techniques: A comparison between ABC and unlabeled antibody (PAP) procedures, J. Kstochem. Cytochem, 29, pp. 577-580, (1981)

[8]

Hun U.S., Chen H.-L., Hu X.-L., Tabibzadeh S., Tumor necrosis factor-a mRNA and protein in human endometrium, Biol Repral, 47, pp. 141-147, (1992)

[9]

Karyia M., Kanzaki H., Takakura K., Imai K., Okamoto N., Emi N., Kariya Y., Mori T., Interieukin-1 inhibits in vitro AyTriimlrretion of human endometrial stromal cells, J. Gin. Endocrinol Metab, 73, pp. 1170-1174, (1991)

[10]

Kauma S., Dennis M., Strom S., Eierman D., Tumer B.S., Interleukin-1/3, human leukocyte antigen HLA-DRa and transforming growth factor-/3 expression in endometrium, placenta and placenta! membranes, Am, J. Obstet. Gynecol, 163, pp. 1430-1437, (1990)

← 1 2 3 4 →