REGULATED IMMUNOGLOBULIN (IG) RNA PROCESSING DOES NOT REQUIRE SPECIFIC CIS-ACTING SEQUENCES - NON-IG RNA CAN BE ALTERNATIVELY PROCESSED IN B-CELLS AND PLASMA-CELLS

被引:52
作者
PETERSON, ML
机构
[1] Dept. of Pathol. and Lab. Medicine, University of Kentucky, College of Medicine, Lexington
[2] Dept. of Pathol. and Lab. Medicine, Univ. of Kentucky Coll. of Medicine, 108A Combs Building, Lexington, KY 40536-0093
关键词
D O I
10.1128/MCB.14.12.7891
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Alternative RNA processing of the heavy-chain immunoglobulin mu gene is regulated during B-cell maturation and requires competition between splice and cleavage-polyadenylation reactions that have balanced efficiencies. Studies with modified mu genes have failed to identify gene-specific sequences required for regulation. Thus, the only important feature for regulation may be the balanced competing splice and cleavage-polyadenylation reactions themselves. If this is so, then alternative RNA processing from any gene with similar competitive RNA processing pathways should also be regulated when expression is compared between B cells and plasma cells. To test this prediction, two nonimmunoglobulin genes engineered to have competing splice and cleavage-polyadenylation reactions were expressed in B cells and plasma cells. The ratios of alternative RNAs produced from both genes are different in the two cell types; like the mu gene, relatively more spliced RNA is produced in B cells than in plasma cells. Also, in a survey of mu gene expression in nine non-B-cell lines, only a T-cell line had an expression pattern similar to that of B cells; the expression patterns of all other lines resembled that of the plasma cells. Therefore, regulated mu RNA processing must be mediated by changes in general processing factors whose activity or abundance is regulated, most likely, in B cells.
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页码:7891 / 7898
页数:8
相关论文
共 57 条
  • [1] SPLICE SITE SELECTION DOMINATES OVER POLY(A) SITE CHOICE IN RNA PRODUCTION FROM COMPLEX ADENOVIRUS TRANSCRIPTION UNITS
    ADAMI, G
    NEVINS, JR
    [J]. EMBO JOURNAL, 1988, 7 (07) : 2107 - 2116
  • [2] SEQUENCE REQUIREMENTS FOR SPLICING OF HIGHER EUKARYOTIC NUCLEAR PRE-MESSENGER-RNA
    AEBI, M
    HORNIG, H
    PADGETT, RA
    REISER, J
    WEISSMANN, C
    [J]. CELL, 1986, 47 (04) : 555 - 565
  • [3] BENECH P, 1985, NUCLEIC ACIDS RES, V13, P1267
  • [4] THE MOLECULAR-BIOLOGY OF IMMUNOGLOBULIN-D
    BLATTNER, FR
    TUCKER, PW
    [J]. NATURE, 1984, 307 (5950) : 417 - 422
  • [5] BROWN SL, 1989, J IMMUNOL, V142, P2072
  • [6] COMPARISON OF INTRON-DEPENDENT AND INTRON-INDEPENDENT GENE-EXPRESSION
    BUCHMAN, AR
    BERG, P
    [J]. MOLECULAR AND CELLULAR BIOLOGY, 1988, 8 (10) : 4395 - 4405
  • [7] REGULATION BY HIV REV DEPENDS UPON RECOGNITION OF SPLICE SITES
    CHANG, DD
    SHARP, PA
    [J]. CELL, 1989, 59 (05) : 789 - 795
  • [8] IMPORTANCE OF INTRONS FOR EXPRESSION OF MOUSE RIBOSOMAL-PROTEIN GENE RPL32
    CHUNG, S
    PERRY, RP
    [J]. MOLECULAR AND CELLULAR BIOLOGY, 1989, 9 (05) : 2075 - 2082
  • [9] A PAUSE SITE FOR RNA POLYMERASE-II IS ASSOCIATED WITH TERMINATION OF TRANSCRIPTION
    ENRIQUEZHARRIS, P
    LEVITT, N
    BRIGGS, D
    PROUDFOOT, NJ
    [J]. EMBO JOURNAL, 1991, 10 (07) : 1833 - 1842
  • [10] ESHHAR Z, 1979, J IMMUNOL, V122, P2430