ELECTRON-TRANSFER ON AN ELECTRODE OF GLUCOSE-OXIDASE IMMOBILIZED IN POLYANILINE

被引：35

作者：

LU, SY ^{[1
]}

LI, CF ^{[1
]}

ZHANG, DD ^{[1
]}

ZHANG, Y ^{[1
]}

MO, ZH ^{[1
]}

CAI, Q ^{[1
]}

ZHU, AR ^{[1
]}

机构：

[1] FUDAN UNIV,SURFACE PHYS LAB,SHANGHAI 200433,PEOPLES R CHINA

来源：

JOURNAL OF ELECTROANALYTICAL CHEMISTRY | 1994年 / 364卷 / 1-2期

关键词：

D O I：

10.1016/0022-0728(93)02928-B

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An electrode composed of glucose oxidase (GOD) immobilized in electropolymerized polyaniline (PANI) on a Pt wire of diameter 0.5 mm was prepared. Scanning tunneling micrographs of both PANI and GOD + PANI showed a wavy long-chain polymer structure, but the latter appeared smoother, thicker and well distributed. The dependences of the oxygen reduction current and the glucose-reduced GOD oxidation current on the glucose concentrations were found from cyclic voltammograms of a GOD + PANI + Pt electrode in glucose solutions. The compound 7-acetyl-10-methyl isoalloxazine (C13H10N4O3) was used as a model of the redox center on the GOD molecule. The results showed that at a potential of -0.3 V/SCE glucose-reduced GOD could be oxidized again and direct electron transfer could occur from the active redox center of the enzyme to the Pt surface through an electron pathway in GOD + PANI and its model electrodes.

引用

页码：31 / 36

页数：6

共 12 条

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