TRANSCRIPTIONAL SILENCER OF THE WILMS-TUMOR GENE WT1 CONTAINS AN ALU REPEAT

被引：63

作者：

HEWITT, SM ^{[1
]}

FRAIZER, GC ^{[1
]}

SAUNDERS, GF ^{[1
]}

机构：

[1] UNIV TEXAS, MD ANDERSON CANC CTR, DEPT BIOCHEM & MOLEC BIOL, HOUSTON, TX 77030 USA

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 1995年 / 270卷 / 30期

关键词：

D O I：

10.1074/jbc.270.30.17908

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Expression of the Wilms' tumor gene WT1 is tightly regulated throughout development. In constrast, the WT1 promoter is promiscuous, functioning in all cell lines tested, We have cloned a transcriptional silencer that is involved in regulation of the WT1 gene. The transcriptional silencer is located in the third intron of the WT1 gene, approximately 12 kilobases from the promoter, and functions to repress transcription from the WT1 promoter in cell lines of non-renal origin. The 460-base pair silencer region is unusual in that it contains a full-length Alu repeat. We have also cloned an enhancer like-element located 1.3 kilobases upstream of the WT1 promoter.

引用

页码：17908 / 17912

页数：5

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