LIPID MODULATION OF NICOTINIC ACETYLCHOLINE-RECEPTOR FUNCTION - THE ROLE OF NEUTRAL AND NEGATIVELY CHARGED LIPIDS

被引：72

作者：

SUNSHINE, C ^{[1
]}

MCNAMEE, MG ^{[1
]}

机构：

[1] UNIV CALIF DAVIS,DEPT BIOCHEM & BIOPHYS,DAVIS,CA 95616

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1992年 / 1108卷 / 02期

关键词：

NICOTINIC ACETYLCHOLINE RECEPTOR; FOURIER TRANSFORM; ION FLUX RESPONSE; PROTEIN LIPID INTERACTION; CHOLESTEROL; AFFINITY-STATE TRANSITION; (T-CALIFORNICA);

D O I：

10.1016/0005-2736(92)90031-G

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effects of negatively charged and neutral lipids on the function of the reconstituted nicotinic acetylcholine receptor from Torpedo californica were determined with two assays using acetylcholine receptor-containing vesicles: the ion flux response and the affinity-state transition. The receptor was reconstituted into three different lipid environments, with and without neutral lipids: (1) phosphatidylcholine/phosphatidylserine; (2) phosphatidylcholine/phosphatidic acid; and (3) phosphatidylcholine/cardiolipin. Analysis of the ion flux responses showed that: (1) all three negatively charged lipid environments gave fully functional acetylcholine receptor ion channels, provided neutral lipids were added; (2) in each lipid environment, the neutral lipids tested were functionally equivalent to cholesterol; and (3) the rate of receptor desensitization depends upon the type of neutral lipid and negatively charged phospholipid reconstituted with the receptor. The functional effects of neutral and negatively charged lipids on the acetylcholine receptor are discussed in terms of protein-lipid interactions and stabilization of protein structure by lipids.

引用

页码：240 / 246

页数：7

共 18 条

[1] EFFECT OF LOCAL-ANESTHETICS ON STEROID-NICOTINIC ACETYLCHOLINE-RECEPTOR INTERACTIONS IN NATIVE MEMBRANES OF TORPEDO-MARMORATA ELECTRIC ORGAN [J].

ARIAS, HR ;

SANKARAM, MB ;

MARSH, D ;

BARRANTES, FJ .

BIOCHIMICA ET BIOPHYSICA ACTA, 1990, 1027 (03) :287-294

[2] LOCALIZATION OF REGIONS OF THE TORPEDO-CALIFORNICA NICOTINIC ACETYLCHOLINE-RECEPTOR LABELED WITH AN ARYL AZIDE DERIVATIVE OF PHOSPHATIDYLSERINE [J].

BLANTON, MP ;

WANG, HH .

BIOCHIMICA ET BIOPHYSICA ACTA, 1991, 1067 (01) :1-8

[3] LIPID-PROTEIN INTERACTIONS IN RECONSTITUTED MEMBRANES CONTAINING ACETYLCHOLINE-RECEPTOR [J].

ELLENA, JF ;

BLAZING, MA ;

MCNAMEE, MG .

BIOCHEMISTRY, 1983, 22 (24) :5523-5535

[4] STABILIZATION OF ACETYLCHOLINE-RECEPTOR SECONDARY STRUCTURE BY CHOLESTEROL AND NEGATIVELY CHARGED PHOSPHOLIPIDS IN MEMBRANES [J].

FONG, TM ;

MCNAMEE, MG .

BIOCHEMISTRY, 1987, 26 (13) :3871-3880

[5] CORRELATION BETWEEN ACETYLCHOLINE-RECEPTOR FUNCTION AND STRUCTURAL-PROPERTIES OF MEMBRANES [J].

FONG, TM ;

MCNAMEE, MG .

BIOCHEMISTRY, 1986, 25 (04) :830-840

[6] TRANSMEMBRANE TOPOLOGY OF ACETYLCHOLINE-RECEPTOR SUBUNITS PROBED WITH PHOTOREACTIVE PHOSPHOLIPIDS [J].

GIRAUDAT, J ;

MONTECUCCO, C ;

BISSON, R ;

CHANGEUX, JP .

BIOCHEMISTRY, 1985, 24 (13) :3121-3127

[7] MULTIPLE SITES OF ACTION FOR NONCOMPETITIVE BLOCKERS ON ACETYLCHOLINE-RECEPTOR RICH MEMBRANE-FRAGMENTS FROM TORPEDO-MARMORATA [J].

HEIDMANN, T ;

OSWALD, RE ;

CHANGEUX, JP .

BIOCHEMISTRY, 1983, 22 (13) :3112-3127

[8] ANNULAR AND NONANNULAR BINDING-SITES FOR CHOLESTEROL ASSOCIATED WITH THE NICOTINIC ACETYLCHOLINE-RECEPTOR [J].

JONES, OT ;

MCNAMEE, MG .

BIOCHEMISTRY, 1988, 27 (07) :2364-2374

[9] RECONSTITUTION OF ACETYLCHOLINE-RECEPTOR FROM TORPEDO-CALIFORNICA WITH HIGHLY PURIFIED PHOSPHOLIPIDS - EFFECT OF ALPHA-TOCOPHEROL, PHYLLOQUINONE, AND OTHER TERPENOID QUINONES [J].

KILIAN, PL ;

DUNLAP, CR ;

MUELLER, P ;

SCHELL, MA ;

HUGANIR, RL ;

RACKER, E .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1980, 93 (02) :409-414

[10] RECONSTITUTION OF ACETYLCHOLINE-RECEPTOR FUNCTION IN MODEL MEMBRANES [J].

MCNAMEE, MG ;

OCHOA, ELM .

NEUROSCIENCE, 1982, 7 (10) :2305-2319

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