EFFECT OF A STABILIZED MICROBUBBLE CONTRAST AGENT ON CW ULTRASOUND INDUCED RED-BLOOD-CELL LYSIS IN-VITRO

被引：46

作者：

MILLER, MW

AZADNIV, M

DOIDA, Y

BRAYMAN, AA

机构：

[1] Department of Biophysics, School of Medicine and Dentristry, University of Rochester, Rochester, New York

[2] Department of Biology, Shiga University of Medical Science, Otsu, Shiga, Seta

来源：

ECHOCARDIOGRAPHY-A JOURNAL OF CARDIOVASCULAR ULTRASOUND AND ALLIED TECHNIQUES | 1995年 / 12卷 / 01期

关键词：

ALBUNEX; ECHO-CONTRAST AGENTS; HUMAN RED BLOOD CELLS; CELL LYSIS;

D O I：

10.1111/j.1540-8175.1995.tb00515.x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Human red blood cells (RBCs) in vitro at various fractional hematocrits (HCTs) were exposed for 60-120 seconds in a dialysis tubing vessel to 1 MHz continuous wave ultrasound (0-5 W/cm2 SPTA intensity); exposure vessels were either rotated at 200 rpm or stationary. Some RBC suspensions also contained Albunex(R) (ALX; a commercially-produced microbubble clinical ultrasound contrast agent) at final concentrations ranging from 0-41 muL/mL RBC suspension. Isonation was either by one transducer or by two opposing, continuously-gated, balanced transducers. For the vessel rotation/no rotation experiments, ultrasound-induced cell lysis was always increased with the ALX regimen relative to that of the no-ALX regimen at HCTs up to about 10%; at higher HCTs (including whole blood), ultrasound-induced hemolysis essectially ceased to occur. The data are consistent with reports of the ineffectiveness of continuous wave ultrasound at 3-5 W/cm2 to lyse cells in vitro at physiological cell densities with or without echo-contrast medium supplementation.

引用

页码：1 / 11

页数：11

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