COMBINED FISH PAINTING AND HARLEQUIN STAINING FOR CELL CYCLE-CONTROLLED CHROMOSOME ANALYSIS IN HUMAN-LYMPHOCYTES

被引：27

作者：

KULKA, U ^{[1
]}

HUBER, R ^{[1
]}

MULLER, P ^{[1
]}

KNEHR, S ^{[1
]}

BAUCHINGER, M ^{[1
]}

机构：

[1] GSF FORSCHUNGSZENTRUM UNWELT & GESUNDHEIT,INST STRAHLENBIOL,D-85758 OBERSCHLEISSHEIM,GERMANY

来源：

INTERNATIONAL JOURNAL OF RADIATION BIOLOGY | 1995年 / 68卷 / 01期

关键词：

D O I：

10.1080/09553009514550881

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We present a simple method that allows scoring of FISH-painted chromosomes exclusively in the first division human lymphocytes. It consists of a combination of FISH with chromosome-specific libraries and a differential sister chromatid staining after BrdU treatment of lymphocyte cultures. The method allows a precise quantification of induced chromosome damage for human biodosimetry.

引用

页码：25 / 27

页数：3

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