Characterization of a cis-Golgi matrix protein, GM130

被引:695
作者
Nakamura, N
Rabouille, C
Watson, R
Nilsson, T
Hui, N
Slusarewicz, P
Kreis, TE
Warren, G
机构
[1] IMPERIAL CANC RES FUND, CELL BIOL LAB, LONDON WC2A 3PX, ENGLAND
[2] UNIV GENEVA, DEPT BIOL CELLULAIRE, CH-1211 GENEVA 4, SWITZERLAND
关键词
D O I
10.1083/jcb.131.6.1715
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Antisera raised to a detergent- and salt-resistant matrix fraction from rat liver Golgi stacks were used to screen an expression library from rat liver cDNA. A full-length clone was obtained encoding a protein of 130 kD (termed GM130), the COOH-terminal domain of which was highly homologous to a Golgi human auto-antigen, golgin-95 (Fritzler et al., 1993). Biochemical data showed that GM130 is a peripheral cytoplasmic protein that is tightly bound to Golgi membranes and part of a larger oligomeric complex. Predictions from the protein sequence suggest that GM130 is an extended rod-like protein with coiled-coil domains, Immunofluorescence microscopy showed partial over-lap with medial- and trans-Golgi markers but almost complete overlap with the cis-Golgi network (CGN) marker, syntaxin5, Immunoelectron microscopy confirmed this location showing that most of the GM130 was located in the CGN and in one or two cisternae on the cis-side of the Golgi stack. GM130 was not re-distributed to the ER in the presence of brefeldin A but maintained its overlap with syntaxin5 and a partial overlap with the ER-Golgi intermediate compartment marker, p53, Together these results suggest that GM130 is part of a cis-Golgi matrix and has a role in maintaining cis-Golgi structure.
引用
收藏
页码:1715 / 1726
页数:12
相关论文
共 64 条
  • [1] GP74 - A MEMBRANE GLYCOPROTEIN OF THE CIS-GOLGI NETWORK THAT CYCLES THROUGH THE ENDOPLASMIC-RETICULUM AND INTERMEDIATE COMPARTMENT
    ALCALDE, J
    EGEA, G
    SANDOVAL, IV
    [J]. JOURNAL OF CELL BIOLOGY, 1994, 124 (05) : 649 - 665
  • [2] BASIC LOCAL ALIGNMENT SEARCH TOOL
    ALTSCHUL, SF
    GISH, W
    MILLER, W
    MYERS, EW
    LIPMAN, DJ
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 1990, 215 (03) : 403 - 410
  • [3] LOCALIZATION OF SED5, A PUTATIVE VESICLE TARGETING MOLECULE, TO THE CIS-GOLGI NETWORK INVOLVES BOTH ITS TRANSMEMBRANE AND CYTOPLASMIC DOMAINS
    BANFIELD, DK
    LEWIS, MJ
    RABOUILLE, C
    WARREN, G
    PELHAM, HRB
    [J]. JOURNAL OF CELL BIOLOGY, 1994, 127 (02) : 357 - 371
  • [4] Beck K. A., 1994, Molecular Biology of the Cell, V5, p240A
  • [5] GOLGI SPECTRIN - IDENTIFICATION OF AN ERYTHROID BETA-SPECTRIN HOMOLOG ASSOCIATED WITH THE GOLGI-COMPLEX
    BECK, KA
    BUCHANAN, JA
    MALHOTRA, V
    NELSON, WJ
    [J]. JOURNAL OF CELL BIOLOGY, 1994, 127 (03) : 707 - 723
  • [6] THE SYNTAXIN FAMILY OF VESICULAR TRANSPORT RECEPTORS
    BENNETT, MK
    GARCIAARRARAS, JE
    ELFERINK, LA
    PETERSON, K
    FLEMING, AM
    HAZUKA, CD
    SCHELLER, RH
    [J]. CELL, 1993, 74 (05) : 863 - 873
  • [7] THE EFFECT OF 1-DEOXYMANNOJIRIMYCIN ON RAT-LIVER ALPHA-MANNOSIDASES
    BISCHOFF, J
    KORNFELD, R
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1984, 125 (01) : 324 - 331
  • [8] BORDIER C, 1981, J BIOL CHEM, V256, P1604
  • [9] A NEW RECOMBINANT DNA STRATEGY FOR THE MOLECULAR-CLONING OF RARE MEMBRANE-PROTEINS
    BRAKE, B
    BRAGHETTA, P
    BANTING, G
    BRESSAN, G
    LUZIO, JP
    STANLEY, KK
    [J]. BIOCHEMICAL JOURNAL, 1990, 267 (03) : 631 - 637
  • [10] CLUETT EB, 1992, J CELL SCI, V103, P773