MOLECULAR-STRUCTURE OF THE HUMAN DESMOPLAKIN-I AND DESMOPLAKIN-II AMINO TERMINUS

被引:84
作者
VIRATA, MLA
WAGNER, RM
PARRY, DAD
GREEN, KJ
机构
[1] NORTHWESTERN UNIV,SCH MED,DEPT PATHOL,CHICAGO,IL 60611
[2] MASSEY UNIV,DEPT PHYS & BIOPHYS,PALMERSTON NORTH,NEW ZEALAND
[3] NORTHWESTERN UNIV,SCH MED,CTR CANC,CHICAGO,IL 60611
关键词
DESMOSOMES; INTERCELLULAR JUNCTIONS; ALTERNATIVE SPLICING;
D O I
10.1073/pnas.89.2.544
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Desmoplakins (DPs) I and II are closely related proteins found in the innermost region of the desmosomal plaque, which serves as a cell surface attachment site for cytoplasmic intermediate filaments. Overlapping cDNA clones comprising 9.2 kilobases of DP-I, predicted to encode a full-length 310-kDa polypeptide (2677 amino acid residues), have now been identified. Here we report the predicted protein sequence and structural analysis of the N terminus of DP, extending our previous study of the rod and carboxyl domains. The N terminus contains groups of heptad repeats that are predicted to form at least two major alpha-helical-rich bundles. Unlike the rod and carboxyl domains, the N terminus did not display a periodic distribution of charged residues. Northern blot mapping and genomic sequence analysis were also undertaken to examine the organization of the DP mRNAs. A 1-kilobase intron was located at the 3' boundary of a DP-I-specific region; however, instead of an intron at the 5' junction, a possible splice donor site was observed within a potential coding sequence, suggesting alternative RNA splicing from an internal donor site.
引用
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页码:544 / 548
页数:5
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