EVIDENCE THAT ALPHA-2-ADRENERGIC PATHWAYS PLAY A MAJOR ROLE IN GROWTH-HORMONE (GH) NEUROREGULATION - ALPHA-2-ADRENERGIC AGONISM COUNTERACTS THE INHIBITORY EFFECT OF MUSCARINIC CHOLINERGIC RECEPTOR BLOCKADE ON THE GH RESPONSE TO GH-RELEASING HORMONE, WHILE ALPHA-2-ADRENERGIC BLOCKADE DIMINISHES THE POTENTIATING EFFECT OF INCREASED CHOLINERGIC TONE ON SUCH STIMULATION IN NORMAL MEN

The aim of this study was to investigate the interrelationships between alpha-2-adrenergic and cholinergic pathways in the control of hypothalamic somatostatin (SRIF) secretion in humans. In eight normal volunteers subjects we compared the pattern of GHRH-induced GH release to that elicited by similar challenge given 60 min after a pretreatment with drugs affecting alpha-2-adrenergic and muscarinic cholinergic neurotransmission. In a control study, synthetic GHRH [GRF-(1-29); 1-mu-g/kg, iv] was administered 60 min after giving placebo. In other experiments, the administration of atropine (1 mg, im), or clonidine (0.300 mg, orally), or atropine plus clonidine, or pyridostigmine (120 mg, orally), or yohimbine (30 mg, orally), or pyridostigmine plus yohimbine, at 0 min was followed by GHRH administration 60 min later. The administration of both clonidine and pyridostigmine significantly (P < 0.01) enhanced the GH responses to GHRH compared to those elicited by this challenge when given after placebo. Conversely, atropine pretreatment significantly (P < 0.01) blocked the GH response to GHRH challenge, whereas yohimbine did not significantly affect it. When atropine and clonidine were given together, the inhibitory effect of the former was overcame and mean GHRH-elicited GH peak response was significantly (P < 0.05) higher than that in the control study. In contrast, pretreatment with yohimbine significantly (P < 0.05) blunted the pyridostigmine-induced enhancement of GHRH-elicited GH release. These data confirm our previous postulate suggesting that the stimulatory effect of clonidine on GH release is mainly exerted by inhibiting the hypothalamic SRIF release. Moreover, the effect of cholinergic neurons on SRIF release seems to be, at least in part, dependent on alpha-2-adrenergic pathways. Based on these data, it can be proposed that the alpha-2-adrenergic system plays a major role in the control of hypothalamic SRIF release, and hence in GH neuroregulation, whereas the muscarinic cholinergic system would participate in such regulation by modulating the functional activity of the former.