TISSUE-SPECIFIC AND HIGH-LEVEL EXPRESSION OF THE HUMAN TYROSINE-HYDROXYLASE GENE IN TRANSGENIC MICE

被引：109

作者：

KANEDA, N

SASAOKA, T

KOBAYASHI, K

KIUCHI, K

NAGATSU, I

KUROSAWA, Y

FUJITA, K

YOKOYAMA, M

NOMURA, T

KATSUKI, M

NAGATSU, T

机构：

[1] NAGOYA UNIV,SCH MED,DEPT BIOCHEM,NAGOYA,AICHI 466,JAPAN

[2] NAGOYA UNIV,RADIOISOTOPE CTR,DIV MED,NAGOYA,AICHI 466,JAPAN

[3] FUJITA HLTH UNIV,SCH MED,INST COMPREHENS MED SCI,TOYOAKE 47011,JAPAN

[4] FUJITA HLTH UNIV,SCH MED,DEPT ANAT,TOYOAKE 47011,JAPAN

[5] CENT INST EXPTL ANIM,DIV DEV BIOTECHNOL,KAWASAKI 213,JAPAN

[6] TOKAI UNIV,SCH MED,DEPT DNA BIOL,ISEHARA,KANAGAWA 25911,JAPAN

来源：

NEURON | 1991年 / 6卷 / 04期

关键词：

D O I：

10.1016/0896-6273(91)90061-4

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Transgenic mice carrying multiple copies of the human tyrosine hydroxylase (TH)gene have been produced. The transgenes were transcribed correctly and expressed specifically in brain and adrenal gland. The level of human TH mRNA in brain was about 50-fold higher than that of endogenous mouse TH mRNA. In situ hybridization demonstrated an enormous region-specific expression of the transgene in substantia nigra and ventral tegmental area. TH immunoreactivity in these regions, though not comparable to the increment of the mRNA, was definitely increased in transgenic mice. This observation was also supported by Western blot analysis and TH activity measurements. However, catecholamine levels in transgenics were not significantly different from those in nontransgenics. These results suggest unknown regulatory mechanisms for human TH gene expression and for the catecholamine levels in transgenic mice.

引用

页码：583 / 594

页数：12

共 44 条

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