FOURIER-TRANSFORM-EPR AND LOW-FREQUENCY-EPR STUDIES OF NITROXIDES

被引：11

作者：

BOWMAN, MK ^{[1
]}

MICHALSKI, TJ ^{[1
]}

PERIC, M ^{[1
]}

HALPERN, HJ ^{[1
]}

机构：

[1] UNIV CHICAGO,MED CTR,CHICAGO,IL 60637

来源：

PURE AND APPLIED CHEMISTRY | 1990年 / 62卷 / 02期

关键词：

D O I：

10.1351/pac199062020271

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The nitroxide spin labels are a class of stable free radicals that have wide applications as probes of chemical and biological systems in electron paramagnetic resonance (EPR) experiments. Measurements of the concentration of paramagnetic species and of rotational or translational motion can be made using the relaxation times and the lineshape of the nitroxide probe. In the fast motional limit, the relaxation times are easily obtained from the conventional EPR spectrum if inhomogeneous broadening from the unresolved hyperfine couplings is not too great. Pulsed and Fourier-Transform EPR (FT-EPR) methods allow the measurements independent of inhomogeneous broadening. In biological studies involving large aqueous samples, it is advantageous to use low-frequency EPR (LF-EPR) near 250 MHz to reduce microwave absorption from the sample. The nitroxide spin-label probes are as effective in LF-EPR as in the usual X-band EPR. © 1990 De Gruyter

引用

页码：271 / 274

页数：4

共 16 条

[1] EFFECT OF TRIPLET SPIN DYNAMICS MEMORY IN ELECTRON-TRANSFER REACTIONS - FOURIER-TRANSFORM ELECTRON-PARAMAGNETIC RESONANCE SPECTROSCOPY [J].