DIFFERENTIATION OF ACANTHAMOEBA STRAINS FROM INFECTED CORNEAS AND THE ENVIRONMENT BY USING RESTRICTION ENDONUCLEASE DIGESTION OF WHOLE-CELL DNA

被引:36
作者
KILVINGTON, S [1 ]
BEECHING, JR [1 ]
WHITE, DG [1 ]
机构
[1] UNIV BATH,SCH BIOL SCI,BATH BA2 7AY,AVON,ENGLAND
关键词
D O I
10.1128/JCM.29.2.310-314.1991
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Restriction endonuclease digestion of Acanthamoeba whole-cell DNA was used to study the relationship between 33 morphologically identical strains from keratitis cases (30 strains), contact lens storage containers (2 strains), and soil (1 strain). Samples digested with BglII, EcoRI, or HindIII and separated by agarose gel electrophoresis contained detectable mitochondrial DNA restriction fragment length polymorphisms (RFLPs). By comparing RFLPs, the strains could be assigned to seven multiple-strain and three single-strain groups. The largest of these contained nine strains, eight of which were isolated in keratitis cases in various locations worldwide and may indicate a group particularly associated with keratitis. Restriction endonuclease analysis of whole-cell DNA is proposed as a valuable technique for detecting mitochondrial DNA RFLPs in the differentiation of morphologically identical Acanthamoeba strains and may therefore be useful in resolving the complex taxonomy of the genus, which has hitherto been founded on subjective morphological criteria.
引用
收藏
页码:310 / 314
页数:5
相关论文
共 35 条
[1]   IMPROVED SELECTIVE ISOLATION OF NAEGLERIA-FOWLERI FROM THE ENVIRONMENT [J].
AUFY, S ;
KILVINGTON, S ;
MANN, PG ;
WARHURST, DC .
TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE, 1986, 80 (02) :350-351
[2]   PATHOGENICITY OF ACANTHAMOEBA AND A CORYNEBACTERIUM IN THE RAT CORNEA [J].
BADENOCH, PR ;
JOHNSON, AM ;
CHRISTY, PE ;
COSTER, DJ .
ARCHIVES OF OPHTHALMOLOGY, 1990, 108 (01) :107-112
[3]   INTERSTRAIN MITOCHONDRIAL-DNA POLYMORPHISM DETECTED IN ACANTHAMOEBA BY RESTRICTION ENDONUCLEASE ANALYSIS [J].
BOGLER, SA ;
ZARLEY, CD ;
BURIANEK, LL ;
FUERST, PA ;
BYERS, TJ .
MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1983, 8 (02) :145-163
[4]   ANALYSIS OF MITOCHONDRIAL-DNA VARIATION AS AN APPROACH TO SYSTEMATIC RELATIONSHIPS IN THE GENUS ACANTHAMOEBA [J].
BYERS, TJ ;
BOGLER, SA ;
BURIANEK, LL .
JOURNAL OF PROTOZOOLOGY, 1983, 30 (02) :198-203
[6]   MEDICAL AND SURGICAL-TREATMENT OF ACANTHAMOEBA KERATITIS [J].
COHEN, EJ ;
PARLATO, CJ ;
ARENTSEN, JJ ;
GENVERT, GI ;
EAGLE, RC ;
WIELAND, MR ;
LAIBSON, PR .
AMERICAN JOURNAL OF OPHTHALMOLOGY, 1987, 103 (05) :615-625
[7]   THE SUITABILITY OF STARCH-GEL ELECTROPHORESIS OF ESTERASES AND ACID-PHOSPHATASES FOR THE STUDY OF ACANTHAMOEBA TAXONOMY [J].
COSTAS, M ;
GRIFFITHS, AJ .
ARCHIV FUR PROTISTENKUNDE, 1980, 123 (03) :272-279
[8]  
COSTAS M, 1983, FEMS MICROBIOL LETT, V17, P231, DOI 10.1111/j.1574-6968.1983.tb00407.x
[9]  
DAVIES DJG, 1988, T BR CONTACT LENS AS, V5, P80
[10]   GROWTH-CHARACTERISTICS, CYTOPATHIC EFFECT IN CELL-CULTURE, AND VIRULENCE IN MICE OF 36 TYPE STRAINS BELONGING TO 19 DIFFERENT ACANTHAMOEBA SPP [J].
DEJONCKHEERE, JF .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1980, 39 (04) :681-685