ISOLATION OF CDNA-ENCODING A NEWLY IDENTIFIED MAJOR ALLERGENIC PROTEIN OF RYE-GRASS POLLEN - INTRACELLULAR TARGETING TO THE AMYLOPLAST

被引：159

作者：

SINGH, MB

HOUGH, T

THEERAKULPISUT, P

AVJIOGLU, A

DAVIES, S

SMITH, PM

TAYLOR, P

SIMPSON, RJ

WARD, LD

MCCLUSKEY, J

PUY, R

KNOX, RB

机构：

[1] UNIV MELBOURNE, SCH BOT, PARKVILLE, VIC 3052, AUSTRALIA

[2] LUDWIG INST CANC RES, MELBOURNE BRANCH, PARKVILLE, VIC 3052, AUSTRALIA

[3] ROYAL MELBOURNE HOSP, WALTER & ELIZA HALL INST MED RES, PARKVILLE, VIC 3050, AUSTRALIA

[4] MONASH UNIV, SCH MED, DEPT PATHOL & IMMUNOL, PRAHRAN, VIC 3181, AUSTRALIA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1991年 / 88卷 / 04期

关键词：

MOLECULAR CLONING; IGE BINDING; LOL PI ALLERGEN; IMMUNOGOLD PROBES; LOLIUM-PERENNE;

D O I：

10.1073/pnas.88.4.1384

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We have identified a major allergenic protein from rye-grass pollen, tentatively designated Lol pIb of 31 kDa and with pI 9.0. A cDNA clone encoding Lol pIb has been isolated, sequenced, and characterized. Lol pIb is located mainly in the starch granules. This is a distinct allergen from Lol pI, which is located in the cytosol. Lol pIb is synthesized in pollen as a pre-allergen with a transit peptide targeting the allergen to amyloplasts. Epitope mapping of the fusion protein localized the IgE binding determinant in the C-terminal domain.

引用

页码：1384 / 1388

页数：5

共 29 条

[1] ANSARI AA, 1989, J BIOL CHEM, V264, P11181
[2] PHYSICOCHEMICAL AND IMMUNOCHEMICAL CHARACTERIZATION OF ALLERGENIC PROTEINS FROM RYE-GRASS (LOLIUM-PERENNE) POLLEN PREPARED BY A RAPID AND EFFICIENT PURIFICATION METHOD
COTTAM, GP
MORAN, DM
STANDRING, R
[J]. BIOCHEMICAL JOURNAL, 1986, 234 (02) : 305 - 310
[3] LIGHT MICROSCOPIC VISUALIZATION OF COLLOIDAL GOLD ON RESIN-EMBEDDED TISSUE
DANSCHER, G
NORGAARD, JOR
[J]. JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1983, 31 (12) : 1394 - 1398
[4] CROSS-REACTIVE AND UNIQUE GRASS GROUP-I ANTIGENIC DETERMINANTS DEFINED BY MONOCLONAL-ANTIBODIES
ESCH, RE
KLAPPER, DG
[J]. JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 1987, 79 (03) : 489 - 495
[5] PREVALENCE OF ASTHMA AND HAY-FEVER IN ENGLAND AND WALES
FLEMING, DM
CROMBIE, DL
[J]. BRITISH MEDICAL JOURNAL, 1987, 294 (6567) : 279 - 283
[6] A CONSERVED CLEAVAGE-SITE MOTIF IN CHLOROPLAST TRANSIT PEPTIDES
GAVEL, Y
VONHEIJNE, G
[J]. FEBS LETTERS, 1990, 261 (02) : 455 - 458
[7] GRIFFITH IJ, 1991, IN PRESS FEBS LETT
[8] A SIMPLE AND VERY EFFICIENT METHOD FOR GENERATING CDNA LIBRARIES
GUBLER, U
HOFFMAN, BJ
[J]. GENE, 1983, 25 (2-3) : 263 - 269
[9] HERRIN D, 1984, PLANT MOL BIOL REP, V2, P24
[10] ALLERGENS FROM COMMON RYE GRASS POLLEN (LOLIUM PERENNE) .2. ALLERGENIC DETERMINANTS AND CARBOHYDRATE MOIETY
JOHNSON, P
MARSH, DG
[J]. IMMUNOCHEMISTRY, 1966, 3 (02): : 101 - &

← 1 2 3 →