AN EASY AND RAPID METHOD FOR ISOLATION OF ENTIRE MYCOBACTERIAL GENOME FOR APPLICATION IN PULSED-FIELD GEL-ELECTROPHORESIS

被引：4

作者：

CHARVIN, M ^{[1
]}

RASTOGI, N ^{[1
]}

LEVYFREBAULT, VV ^{[1
]}

机构：

[1] INST PASTEUR, UNITE TB & MYCOBACTERIES, F-75724 PARIS 15, FRANCE

来源：

CURRENT MICROBIOLOGY | 1991年 / 22卷 / 05期

关键词：

D O I：

10.1007/BF02091963

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A DNA extraction suitable for mycobacterial lysis in gentle conditions compatible with genome analysis by pulsed-field gel electrophoresis is presented. Effects of preliminary treatments with SDS, Triton X-100, and hexane on mycobacterial outer layer were observed by electron microscopy. The most efficient procedure, performed on cells from liquid or solid medium, consisted of treatment by Triton X-100, agarose embedding of the cells, and further treatment with alpha-amylase followed by lysozyme and SDS-proteinase K.

引用

页码：327 / 331

页数：5

共 8 条

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