CATALYTIC ACTIVITY AND IMMUNOHISTOCHEMICAL LOCALIZATION OF FLAVIN-CONTAINING MONOOXYGENASE IN RAT-KIDNEY

被引：3

作者：

BHAMRE, S

SHANKAR, SK

BHAGWAT, SV

RAVINDRANATH, V

机构：

[1] NATL INST MENTAL HLTH & NEUROSCI,DEPT NEUROCHEM,HOUSUR RD,BANGALORE 560029,KARNATAKA,INDIA

[2] NATL INST MENTAL HLTH & NEUROSCI,DEPT NEUROPATHOL,BANGALORE 560029,KARNATAKA,INDIA

来源：

LIFE SCIENCES | 1993年 / 52卷 / 20期

关键词：

D O I：

10.1016/0024-3205(93)90040-A

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

The presence of flavin-containing monooxygenase activity was examined in rat kidney microsomes using N,N-dimethylaniline and methimazole as substrates. Western immunoblot analysis using antisera to porcine liver and rabbit lung flavin-containing monooxygenase indicated immunological cross-reactivity between rat kidney, porcine liver and rabbit lung flavin-containing monooxygenase. Immunohistochemical studies using antisera to rabbit lung flavin-containing monooxygenase demonstrated localization of this enzyme in the proximal and distal convoluted tubules of the renal cortex, the collecting ducts in the renal medulla, but not the glomeruli. This observation indicates the co-localization of flavin-containing monooxygenase and cytochrome P-450 in the metabolically active and absorptive compartment of the renal parenchyma.

引用

页码：1601 / 1607

页数：7

共 18 条

[1] PRESENCE OF FLAVIN-CONTAINING MONOOXYGENASE IN RAT-BRAIN [J].