CHEMILUMINESCENCE REACTION OF THIAZIDE COMPOUNDS WITH TRIS(2,2'-BIPYRIDINE) RUTHENIUM(III)

The chemiluminescence reaction of five thiazide diuretic compounds with tris(2,2'-bipyridine)ruthenium(III) is studied by flow injection over a range of pH values of the carrier stream. The optimum pH range was generally 4-6. One compound, hydroflumethiazide, could be detected at the 0.06 pmole (1 ng ml-1) level. The detection limits for hydrochlorothiazide and cyclothiazide were at 1-2 pmole (13 and 41 ng ml-1, respectively). Two other compounds, chlorothiazide and trichlormethiazide, are only detectable at mug ml-1 levels. Hydrochlorothiazide was quantified with a 103% recovery from a pharmaceutical capsule.