THE BETA-SUBUNIT RIF-CLUSTER-I IS ONLY ANGSTROMS AWAY FROM THE ACTIVE-CENTER OF ESCHERICHIA-COLI RNA-POLYMERASE

被引：50

作者：

SEVERINOV, K

MUSTAEV, A

SEVERINOV, E

KOZLOV, M

DARST, SA

GOLDFARB, A

机构：

[1] PUBL HLTH RES INST,NEW YORK,NY 10016

[2] ROCKEFELLER UNIV,NEW YORK,NY 10021

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 1995年 / 270卷 / 49期

关键词：

D O I：

10.1074/jbc.270.49.29428

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Ribonucleotide analogs bound in the initiating site of Escherichia coli RNA polymerase-promoter complex were cross-linked to the beta subunit. Using limited proteolysis and chemical degradation, the cross-link was mapped to a segment of beta between amino acids Val(516) and Arg(540). This region (Rif-cluster I) is known to harbor many rifampicin-resistant (Rif(R)) mutations. The results demonstrate that Rif-cluster I is part of the ''5'-face'' of the active center and provide structural basis for the long-known effects of Rif(R) mutations on transcription initiation, elongation, and termination.

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页码：29428 / 29432

页数：5

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